75937-15-4

Usage

Uses

Used in Synthesis of Biotinylated Thyroxine Conjugates:
Acetamide, N-[2-[(2,5-dioxo-1-pyrrolidinyl)oxy]-1-[[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]methyl]-2-oxoethyl]-, (S)is used as a key intermediate in the synthesis of conjugates containing the residues of vitamin H (biotin) and thyroid hormone thyroxine. These biotinylated thyroxine conjugates serve as bifunctional ligands, which can be utilized in the development of a test system for a lanthanide immunofluorometric assay.
Used in Diagnostic Applications:
In the medical and diagnostic industry, Acetamide, N-[2-[(2,5-dioxo-1-pyrrolidinyl)oxy]-1-[[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]methyl]-2-oxoethyl]-, (S)plays a crucial role in the development of a sensitive and accurate test system for measuring free thyroxine levels in human serum. This test system is based on a lanthanide immunofluorometric assay, which takes advantage of the unique properties of biotinylated thyroxine conjugates to provide reliable results for thyroid function assessment.

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 26041-51-0 (S)-2-acetamido-3-(4-(4-hydroxy-3,5-diaiodophenoxy)-3,5-diaiodophenyl)propanoic acid 
• 51-48-9 L-thyroxine 

Relevant academic research and scientific papers

Preparation method of hapten acetyl iodothyroxine active coupling reagent

The invention discloses a preparation method of a hapten acetyl iodothyroxine active coupling reagent. The preparation method comprises the following steps: preparing acetic acid active ester; subjecting the prepared acetic acid active ester to reacting with iodothyroxine to synthesize an intermediate compound 1; and subjecting the intermediate compound 1 to reacting with N-hydroxysuccinimide and a condensing agent to synthesize the hapten acetyl iodothyroxine active coupling reagent. The preparation method has the advantages of low price and easy availability of raw materials, few synthesis steps, simplicity in operation, convenience in extraction and purification and high yield, provides a brand new solution for synthesizing the hapten acetyl iodothyroxine active coupling reagent, and overcomes problems in synthesis of artificial antigen key raw materials during preparation of monoclonal antibodies from micromolecule haptens and in synthesis of key raw materials in development of kits for clinical detection of thyroid function items.

An Easy Preparation of Hapten Active Esters via Solid Supported EDAC

Bioconjugates are preferably prepared by reacting an active ester of the hapten of interest to the protein.Preparation of active esters with solid supported EDAC and N-hydroxysuccinimide or pentafluorophenol affords active esters in excellent yield and pu