79642-50-5

Usage

Uses

Used in Biochemistry Research:
DSG is used as a membrane-soluble, amine-reactive N-hydroxysuccinamide ester-based homobifunctional crosslinking reagent. It aids in the isolation of specific protein complexes, such as the insulin receptor, by covalently linking them together. This enhances the stability and detection of these complexes during experimental procedures.

Characteristics

Features of disuccinimidyl glutarate:Reactive groups: NHS ester (both ends)Reactive towards: amino groups (primary amines)Non-cleavableWater-insoluble (dissolve first in DMF or DMSO)Membrane-permeable, allowing for intracellular crosslinkingCan increase crosslinking efficiency compared to DSS in some applications

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 110-94-1 1,5-pentanedioic acid 
• 2873-74-7 gloutaric dichloride 

Downstream Products

• 1307864-43-2 NSP-DMAE-ZCB-NHS 

Relevant academic research and scientific papers

MODIFIED THERAPEUTIC AGENTS AND COMPOSITIONS THEREOF

Methods and compositions are provided for extending the half-life of a therapeutic agent. A modified therapeutic agent (mTA) comprises a therapeutic agent, a staple, and a half-life extending molecule. The mTAs disclosed herein may be used to treat a disease or a condition in a subject in need thereof.

Preparation and properties of gelatin films incorporated with N-hydroxysuccinimide-activated end-bit binary acid

A series of novel cross-linkers, N-hydroxysuccinimide (NHS)-activated end-bit binary acid (NHS-C4, C5, C6, C8, C10, C14), were synthesised to modify gelatin films and the crosslinking effects were compared. Homogeneous films with the exception of the film crosslinked by NHS-C14 were observed and the thickness was measured using a scanning electron microscope. The section feature influenced by different film-treatment conditions was also recorded. The differential scanning calorimetry results indicated higher thermal stability. The water contact angles confirmed enhanced hydrophobicity. NHS-C6, which was used as a probe crosslinker, exhibited the best crosslinking effect that the content of the free -NH2 achieved was the lowest out of all the crosslinkers. The biodegradation results of gelatin films modified by NHS-C6 exhibited better degradation-resistance and excellent stability. In addition, the optimal experimental conditions were 45°C for 12 h when [NHS-C6]/[-NH2] = 2.5.

3D small-molecule microarrays

A PEG based 3D hydrogel slide was developed specifically for small-molecule microarraying purposes, which displayed improved loading capacity, signal sensitivity and spot morphology compared with a commercially available slide and comparative 2D slide. The Royal Society of Chemistry 2009.

Double strand DNA cleavage with a binuclear iron complex

Covalently linking two single strand DNA cleaving agents resulted in a new biomimetic binuclear iron complex capable of effecting oxidative double strand DNA cleavage. The Royal Society of Chemistry.