80206-32-2

Basic information

• Product Name: (2R,3R)-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate
• Synonyms: (2R,3R)-2,3-Dihydroxy-4-oxobutyl dihydrogen phosphate; butanal, 2,3-dihydroxy-4-(phosphonooxy)-, (2R,3R)-
• CAS NO: 80206-32-2
• Molecular Formula: C₄H₉O₇P
• Molecular Weight: 200.0838
• Mol File: 80206-32-2.mol
• Article Data: 2

Chemical Properties

• Boiling Point: 482.5°C at 760 mmHg
• Flash Point: 245.6°C
• Density: 1.768g/cm³
• Vapor Pressure: 1.24E-10mmHg at 25°C
• Refractive Index: 1.538
• CAS DataBase Reference: (2R,3R)-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate(CAS DataBase Reference)
• NIST Chemistry Reference: (2R,3R)-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate(80206-32-2)
• EPA Substance Registry System: (2R,3R)-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate(80206-32-2)

Safety Data

• Hazardous Substances Data: 80206-32-2(Hazardous Substances Data)

Upstream product

• 56-73-5 D-glucose 6-phosphate 
• 7732-18-5 water 
• 546-67-8 lead(IV) tetraacetate 
• 5996-17-8 O⁶-phosphono-D-glucose; potassium-salt 
• 64-19-7 acetic acid 
• 802294-64-0 propionic acid 
• 41452-29-3 D-fructose-6-phosphoric acid 
• 643-13-0 D-fructose-6-phosphate 

Relevant articles and documents

Cloning, expression, purification, cofactor requirements, and steady state kinetics of phosphoketolase-2 from Lactobacillus plantarum

The genes xpk1 and xpk2(Δ1-21) encoding phosphoketolase-1 and (Δ1-7)-truncated phosphoketolase-2 have been cloned from Lactobacillus plantarum and expressed in Escherichia coli. Both gene-products display phosphoketolase activity on fructose-6-phosphate in extracts. A N-terminal His-tag construct of xpk2(Δ1-21) was also expressed in E. coli and produced active His-tagged (Δ1-7)-truncated phosphoketolase-2 (hereafter phosphoketolase-2). Phosphoketolase-2 is activated by thiamine pyrophosphate (TPP) and the divalent metal ions Mg2+, Mn2+, or Ca2+. Kinetic analysis and data from the literature indicate the activators are MgTPP, MnTPP, or CaTPP, and these species activate by an ordered equilibrium binding pathway, with Me2+TPP binding first and then fructose-6-phosphate. Phosphoketolase-2 accepts either fructose-6-phosphate or xylulose-5-phosphate as substrates, together with inorganic phosphate, to produce acetyl phosphate and either erythrose-4-phosphate or glyceraldehyde-3-phosphate, respectively. Steady state kinetic analysis of acetyl phosphate formation with either substrate indicates a ping pong kinetic mechanism. Product inhibition patterns with erythrose-4-phosphate indicate that an intermediate in the ping pong mechanism is formed irreversibly. Background mechanistic information indicates that this intermediate is 2-acetyl-TPP. The irreversibility of 2-acetyl-TPP formation might explain the overall irreversibility of the reaction of phosphoketolase-2.