85196-33-4Relevant academic research and scientific papers
EGFR-targeting fluorescence quenching probe and preparation method and application thereof
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Paragraph 0064; 0081-0085, (2020/12/09)
The invention relates to the technical field of molecular probes, and discloses an EGFR-targeting fluorescence quenching probe and a preparation method and application thereof. The fluorescence quenching probe structurally comprises a pharmacodynamic skeleton structure, a fluorescence quenching group and a fluorescence labeling group, wherein the fluorescence labeling group is selected from BODIPYFL dye; and the fluorescence quenching group is selected from 2, 4-dinitrophenol. The pharmacodynamic skeleton structure can be combined with EGFR protein in a targeting mode, and when the quenchinggroup in the molecular probe leaves, the fluorescence labeling group can conduct specific tracing and imaging; and by means of molecular imaging, the expression process of specific cells and moleculescan be observed in real time, a target spot is traced, and therefore disease development status evaluation and accurate detection of EGFR mutation typing of lung cancer patients are achieved at the molecular pathological level.
A general approach for the development of fluorogenic probes suitable for no-wash imaging of kinases in live cells
Zhang, Qing,Liu, Hui,Pan, Zhengying
supporting information, p. 15319 - 15322 (2015/01/08)
A general approach is presented for developing small molecule-based fluorogenic probes suitable for no-wash imaging of endogenous kinases in live cells. Probe 1, including a fluorophore-quencher system, was only turned on upon reacting with its target kinase Btk, and disclosed Btk's cellular location in live cells without any washing.
