85231-43-2

Upstream product

• 80817-46-5 bis(benzotriazol-1-yl)(2-chlorophenyl)phosphate 
• 64325-78-6 N₆-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine 
• 91592-78-8 N⁽⁶⁾-benzoyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)adenosine 3'-(benzotrizol-1-yl 2-chlorophenylphosphate) 
• 50-89-5 thymidine 
• 57064-86-5 O^3'-methoxyacetyl-thymidine 
• 94936-21-7 C₄₈H₄₆ClN₆O₈P 
• 98120-31-1 C₅₇H₅₅ClN₇O₁₅P 

Downstream Products

• 85240-02-4 C₆₀H₅₅Cl₂N₇O₁₆P₂ 
• 91592-69-7 C₆₆H₅₈Cl₂N₁₀O₁₆P₂ 

Relevant academic research and scientific papers

INTRODUCTION OF NONAQUEOUS OXIDATION PROCEDURE IN THE PHOSPHITE TRIESTER ROUTE FOR OLIGONUCLEOTIDE SYNTHESIS

Iodobenzene diacetate 5 or tetrabutylammonium periodate 6 oxidize phosphite into phosphate under nonaqueous conditions and can be conveniently used in oligodeoxynucleotide synthesis via the phosphite triester route.

SYNTHESIS OF DNA FRAGMENTS BY THE HYDROXYBENZOTRIAZOLE PHOSPHOTRIESTER APPROACH

The application of the phosphorylating agent 2-chlorophenyl-o,o-bis--phosphate (HOBT-approach) for the introduction of 3'-5'-phosphotriester linkages between a properly 5'-protected (4,4'-dimethoxytrityl) deoxy-nucleoside and a deoxy-nucleoside having free OH groups is described.In this way, all sixteen partially-protected DNA dimers having a free 3'-OH group have been synthesized.The dimers thus obtained have been applied, using the HOBT-approach, to the synthesis of the DNA fragments d-CGCGCG, d-CGTACG and d-ATATTAATAAC.