867259-20-9Relevant academic research and scientific papers
Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety
Ueno, Yoshihito,Kawamura, Akihiro,Takasu, Keiji,Komatsuzaki, Shinji,Kato, Takumi,Kuboe, Satoru,Kitamura, Yoshiaki,Kitade, Yukio
experimental part, p. 2761 - 2769 (2009/09/08)
This paper describes the synthesis and properties of a novel molecular beacon (MB) containing a benzene-phosphate backbone at its stem moiety. The fluorescence intensity of MBs was found to stabilize by the introduction of the benzene-phosphate backbone at its stem moiety. Furthermore, an MB containing the benzene-phosphate backbone was more resistant to DNase I (endonuclease) than an MB comprising natural DNA and 2′-O-methyl-RNA. These results indicate that the MB with the benzene-phosphate backbone is superior as a molecular beacon as compared to the MB composed of natural DNA and 2′-O-methyl-RNA. The Royal Society of Chemistry 2009.
Synthesis and properties of nucleic acid analogues consisting of a benzene-phosphate backbone
Ueno, Yoshihito,Kato, Takumi,Sato, Kumiko,Ito, Yasutomo,Yoshida, Mahito,Inoue, Takumi,Shibata, Aya,Ebihara, Masahiro,Kitade, Yukio
, p. 7925 - 7935 (2007/10/03)
The synthesis and properties of a nucleic acid analogue consisting of a benzene-phosphate backbone are described. The building blocks of the nucleic acid analogue are composed of bis(hydroxymethyl)-benzene residues connected to nucleobases via the biaryl-like axis. Stabilities of the duplexes were studied by thermal denaturation. It was found that the thermal stabilities of the duplexes composed of the benzene-phosphate backbone are highly dependent on their sequences. The duplexes with the benzene-phosphate backbone comprised of the mixed sequences were thermally less stable than the natural DNA duplexes, whereas that composed of the homopyrimidine and homopurine sequences was thermally and thermodynamically more stable than the corresponding natural DNA duplex. It was suggested that the analogues more efficiently stabilize the duplexes in a B-form duplex rather than in an A-form duplex. Thus, the duplexes consisting of the benzene-phosphate backbone, especially composed of the homopyrimidine and homopurine sequences, may offer a novel structural motif useful for developing novel materials applicable in the fields of bio- and nanotechnologies.
