87333-22-0Relevant articles and documents
Synthesis of N-acetyl-S-(3-coumarinyl)-cysteine methyl ester and HPLC analysis of urinary coumarin metabolites
Eisenbrand, Gerhard,Otteneder, Michael,Tang, Weici
, p. 249 - 258 (2008/01/27)
N-Acetyl-S-(3-coumarinyl)cysteine, a metabolite of coumarin in rodents, has been synthesized as methyl ester. A new synthetic route to prepare N-acetyl-S-(3-coumarinyl)-D,L-cysteine methyl ester (1) comprises reaction of 3-mercaptocoumarin (3) with N-acetyl-3-chloro-D,L-alanine methyl ester (4). N-acetyl-S-(4-coumarinyl)-L-cysteine (10) was obtained by reaction of 3-bromocoumarin (12) and N-acetyl-L-cysteine (13). A method for the determination of N-acetyl-S-(3-coumarinyl)cysteine as its methyl ester in urine by HPLC has been developed.