879-50-5Relevant academic research and scientific papers
Synthesis, pharmacological evaluation and docking studies of pyrrole structure-based CB2 receptor antagonists
Ragusa, Giulio,Gómez-Ca?as, María,Morales, Paula,Hurst, Dow P.,Deligia, Francesco,Pazos, Ruth,Pinna, Gerard A.,Fernández-Ruiz, Javier,Goya, Pilar,Reggio, Patricia H.,Jagerovic, Nadine,García-Arencibia, Moisés,Murineddu, Gabriele
, p. 651 - 667 (2015)
Abstract During the last years, there has been a continuous interest in the development of cannabinoid receptor ligands that may serve as therapeutic agents and/or as experimental tools. This prompted us to design and synthesize analogues of the CB2 receptor antagonist N-fenchyl-5-(4-chloro-3-methyl-phenyl)-1-(4-methyl-benzyl)-1H-pyrazole-3-carboxamide (SR144528). The structural modifications involved the bioisosteric replacement of the pyrazole ring by a pyrrole ring and variations on the amine carbamoyl substituents. Two of these compounds, the fenchyl pyrrole analogue 6 and the myrtanyl derivative 10, showed high affinity (Ki in the low nM range) and selectivity for the CB2 receptor and both resulted to be antagonists/inverse agonists in [35S]-GTPγS binding analysis and in an in vitro CB2 receptor bioassay. Cannabinoid receptor binding data of the series allowed identifying steric constraints within the CB2 binding pocket using a study of Van der Waals' volume maps. Glide docking studies revealed that all docked compounds bind in the same region of the CB2 receptor inactive state model.
