90457-20-8Relevant academic research and scientific papers
Bioimaging of peroxynitrite in MCF-7 Cells by a new fluorescent probe rhodamine b phenyl hydrazide
Ambikapathi, Gopi,Kempahanumakkagari, Suresh Kumar,Ramappa Lamani, Babu,Kuramkote Shivanna, Devaraju,Bodagur Maregowda, Harish,Gupta, Anushree,Malingappa, Pandurangappa
, p. 705 - 712 (2013)
Peroxynitrite is a potent oxidizing and nitrating agent which has detrimental effects on cells by altering the structure and function of biomolecules present within. A fluorescent probe rhodamine B phenyl hydrazide (RBPH) has been proposed for peroxynitrite (ONOO-) imaging in MCF-7 cells based on its oxidation property, which converts RBPH to pink colored and highly fluorescent rhodamine B. The fluorescence emission intensity of the rhodamine B produced in the above process is linearly related to the concentration of peroxynitrite. The method obeys Beer's law in the concentration range 2-20 nM and the detection limit has been found to be 1.4 nM. The possible reaction mechanism of peroxynitrite with RBPH to produce rhodamine B has been discussed with spectroscopic evidence. The Probe is selective to the peroxynitrite in the pH range 6-8 which is near physiological pH. Fluorescence microscopic studies suggest that the probe is cell permeable and hence peroxynitrite was imaged in MCF-7 cells.
A fluorescent chemodosimeter for Hg2+based on a spirolactam ring-opening strategy and its application towards mercury determination in aqueous and cellular media
Kumar, Kempahanumakkagaari Suresh,Ramakrishnappa, Thippeswamy,Balakrishna, R. Geetha,Pandurangappa, Mallingappagari
, p. 67 - 74 (2014)
A novel fluorescent chemosensor rhodamine B phenyl hydrazide (RBPH) for Hg2+ was designed and synthesized. This probe is highly sensitive, selective, and irreversible for Hg2+ and exhibits fluorescent response at 580 nm. RBPH also displayed detectable color change from colorless to pink upon treatment with Hg2+. This property has been utilized as naked eye detection for Hg2+ in various industrial samples. Fluorescence microscopic experiments demonstrated that this chemosensor is cell permeable and can be used for fluorescence imaging of Hg2+ in cellular media. This probe can detect Hg2+ with good linear relationships from 1 to 100 nM with r = 0.99983 and the limit of detection were found to be 0.019 nM with ± 0.91 % RSD at 10 nM concentrations.
Rhodamine-based derivatives for Cu2+ sensing: Spectroscopic studies, structure-recognition relationships and its test strips
Yang, Yunxu,Gao, Weiling,Sheng, Ruilong,Wang, Weili,Liu, Hui,Yang, Wumin,Zhang, Tianyi,Zhang, Xuetao
, p. 14 - 20 (2011)
A rhodamine spirolactam/2-hydrazinopyridine derivative was synthesized and characterized, which exhibited high selectivity to Cu2+ over other metal cations. The Cu2+ recognition of this rhodamine derivative could be detected by fluor
Turn-on type fluorogenic and chromogenic probe for the detection of trace amount of nitrite ion in water
Saleem, Muhammad,Abdullah, Razack,Hong, In Seok,Lee, Ki-Hwan
, p. 389 - 393 (2013/08/25)
A rhodamine B-based fluorescent probe for nitrite ion (NO2 ) has been designed, synthesized, characterized and its properties for recognition of NO2 were studied. Nearly non fluorescent probe upon reaction with nitrite ion significantly triggered the fluorescence. Fluorescence response is based on ring opening of the spirolactam of rhodamine B phenyl hydrazide showing maximum absorbance at 552 nm and maximum emission at 584 nm. Probe 3 exhibited high sensitivity and extreme selectivity for nitrite ion over other common ions and oxidants (Cl, ClO, ClO2 , ClO3 , ClO4 , SO4 2, SiO3 2, NO3 2, CO3 2) examined in methanol water (1:1, v/v) at pH 7.0. The probe might be a new efficient tool for detection of nitrite ion in natural water and biological system.
