908007-17-0

Basic information

• Product Name: N-(3-Azidopropyl)biotinaMide
• Synonyms: N-(3-Azidopropyl)biotinaMide;Biotin-azide;N-(3-Azidopropyl)biotinamide
• CAS NO: 908007-17-0
• Molecular Formula: C₁₃H₂₂N₆O₂S
• Molecular Weight: 326.41778
• EINECS: -0
• Mol File: 908007-17-0.mol
• Article Data: 18

Chemical Properties

• Melting Point: 166.0 to 170.0 °C
• Boiling Point: No Data Availablemg
• Appearance: /
• CAS DataBase Reference: N-(3-Azidopropyl)biotinaMide(CAS DataBase Reference)
• NIST Chemistry Reference: N-(3-Azidopropyl)biotinaMide(908007-17-0)
• EPA Substance Registry System: N-(3-Azidopropyl)biotinaMide(908007-17-0)

Safety Data

• Hazardous Substances Data: 908007-17-0(Hazardous Substances Data)

Usage

Uses

N-(3-Azidopropyl)biotinamide is used as a reactant in the synthesis of clickable coumarins as fluorescent labels for amino acids.

Upstream product

• 58-85-5 biotin 
• 35013-72-0 biotin N-Hydroxysuccinimide ester 
• 72320-38-8 3-azidopropan-1-ol 
• 88192-19-2 3-azidopropylamine 

Relevant articles and documents

Site-specific protein modification through CuI-catalyzed 1,2,3-triazole formation and its implementation in protein microarray fabrication

(Figure Presented) Out of site: A protein expression system was combined with CuI-catalyzed 1,2,3-triazole formation to modify a target protein at its C terminus. The immobilized core protein can be modified by diverse small molecules in a site

Photoaffinity Probe Reveals the Potential Target of Harringtonolide for Cancer Cell Migration Inhibition

Harringtonolide (HO, 1) is a bioactive diterpenoid tropone isolated from Cephalotaxus harringtonia with antiproliferation activity. Until now there have been no reports to elucidate its anticancer mechanism. Herein we report the synthesis of HO-derived probes (10, 11, and 12) to identify the possible target of HO. As a result, the application of a novel photoaffinity alkyne-tagged probe from HO (compound 12) showed direct engagement between HO and receptor for activated C kinase 1 (RACK1). Furthermore, HO could suppress the epithelial-mesenchymal transition (EMT) process and inhibit activation of the FAK/Src/STAT3 signaling pathway in A375 cells. This study provides a groundwork for HO as an effective antitumor agent that targets RACK1 to suppress cancer cell migration.

Chemical Probes Reveal Sirt2's New Function as a Robust "eraser" of Lysine Lipoylation

Lysine lipoylation, a highly conserved lysine post-translational modification, plays a critical role in regulating cell metabolism. The catalytic activity of a number of vital metabolic proteins, such as pyruvate dehydrogenase (PDH), depends on lysine lipoylation. Despite its important roles, the detailed biological regulatory mechanism of lysine lipoylation remains largely unexplored. Herein we designed a powerful affinity-based probe, KPlip, to interrogate the interactions of lipoylated peptide/proteins under native cellular environment. Large-scale chemical proteomics analysis revealed a number of binding proteins of KPlip, including sirtuin 2 (Sirt2), an NAD+-dependent protein deacylase. To explore the potential activity of Sirt2 toward lysine lipoylation, we designed a single-step fluorogenic probe, KTlip, which reports delipoylation activity in a continuous manner. The results showed that Sirt2 led to significant delipoylation of KTlip, displaying up to a 60-fold fluorescence increase in the assay. Further kinetic experiments with different peptide substrates revealed that Sirt2 can catalyze the delipoylation of peptide (DLAT-PDH, K259) with a remarkable catalytic efficiency (kcat/Km) of 3.26 × 103 s-1 M-1. The activity is about 400-fold higher than that of Sirt4, the only mammalian enzyme with known delipoylation activity. Furthermore, overexpression and silencing experiments demonstrated that Sirt2 regulates the lipoylation level and the activity of endogenous PDH, thus unequivocally confirming that PDH is a genuine physiological substrate of Sirt2. Using our chemical probes, we have successfully established the relationship between Sirt2 and lysine lipoylation in living cells for the first time. We envision that such chemical probes will serve as useful tools for delineating the roles of lysine lipoylation in biology and diseases.

Alkynyl sugar analogs for labeling and visualization of glycoconjugates in cells

Methods for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates are disclosed. Alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars are incorporated into cellular glycoconjugates. Chemical probes comprising an azide group and a visual or fluorogenic probe and used to label alkyne-derivatized sugar-tagged glycoconjugates are disclosed. Chemical probes bind covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and are visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA, confocal microscopy, and mass spectrometry.