912641-81-7Relevant academic research and scientific papers
CROSSLINKED MATERIALS
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Paragraph 160, (2020/05/28)
The present application describes to the synthesis, formulation and uses of crosslinkable entities and crosslinked materials.
Electrochemical luminescent probe (tris(bipyridine) ruthenium-CBT) as well as preparation method and application thereof
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Paragraph 0074-0081, (2017/12/06)
The invention discloses an electrochemical luminescent probe (tris(bipyridine) ruthenium-CBT) as well as a preparation method and application thereof, and relates to the technical field of biological detection. The method comprises the following steps: leading an active amino group into CABT, so as to obtain active amino-CBT, wherein the active amino-CBT is modified on tris(bipyridine) ruthenium; modifying the active amino-CBT on Ru(bpy)3 via dehydration condensation of the amino group and a carboxy group, so as to obtain an electrochemical luminescent probe (Ru(bpy)3-CBT). The method is simpler and faster and is good in universality; different target proteases can be detected by changing corresponding polypeptide substrate sequences; the application range is wide; the method is good in specificity of detection for the proteases and high in sensitivity. The probe is simple in design and short in operation steps, therefore, the probe is popularized and applied easily in the fields of scientific research, clinical diagnosis and the like; a detection strategy is simple; special material modification and processing requirements are avoided.
Sequence-specific 2-cyanobenzothiazole ligation
Ramil, Carlo P.,An, Peng,Yu, Zhipeng,Lin, Qing
supporting information, p. 5499 - 5502 (2016/05/24)
The use of small, natural chemical reporters in conjunction with catalyst-free bioorthogonal reactions will greatly streamline protein labeling in a cellular environment with minimum perturbation to their function. Here we report the discovery of a 2-cyan
Site-specific immobilization of biomolecules by a biocompatible reaction between terminal cysteine and 2-cyanobenzothiazole
Wang, Ping,Zhang, Chong-Jing,Chen, Ganchao,Na, Zhenkun,Yao, Shao Q.,Sun, Hongyan
supporting information, p. 8644 - 8646 (2013/09/23)
We report herein a new site-specific microarray immobilization method based on a biocompatible reaction between terminal cysteine and 2-cyanobenzothiazole (CBT). This immobilization strategy has been successfully applied to anchor small molecules, peptides and proteins onto microarrays.
Controlling intracellular macrocyclization for the imaging of protease activity
Ye, Deju,Liang, Gaolin,Ma, Man Lung,Rao, Jianghong
supporting information; experimental part, p. 2275 - 2279 (2011/04/22)
(Figure Presented) Reporters on the ground: An intramolecular macrocyclization reaction took place highly efficiently in live cells under the control of a specific enzyme and reduction by glutathione (GSH; see picture). Macrocyclic products (represented as blue rings) synthesized in cells self-assembled into nanoparticles aggregated and retained at the site near the enzyme location to report local proteolytic activity in live cells.
A biocompatible condensation reaction for the labeling of terminal cysteine residues on proteins
Ren, Hongjun,Xiao, Fei,Zhan, Ke,Kim, Young-Pil,Xie, Hexin,Xia, Zuyong,Rao, Jianghong
supporting information; experimental part, p. 9658 - 9662 (2010/04/28)
Going live: A protein-labeling method based on the use of a single amino acid taglan N-terminal cysteine residuel and small-molecule probes containing a cyanobenzothiazole (CBT) unit has been used for the specific fluorescence labeling of proteins in vitro and at the surface of live cells (see scheme). This simople ligation reaction proceeds with a high degree of specificity under physiological conditions. Rd: a rhodamine dye; TEV: Tobacco etch virus.
