91592-58-4Relevant academic research and scientific papers
Efficient and flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry
Zehl, Andrea,Starke, Antje,Cech, Dieter,Hartsch, Thomas,Merkl, Rainer,Fritz, Hans-Joachim
, p. 2677 - 2678 (2007/10/03)
An efficient synthetic approach to protected trinucleotide phosphoramidites suitable for codon-by-codon synthesis of structural gene combinatorial libraries is described; the procedure rests on the use of a pair of orthogonal protecting groups for the two
SYNTHESIS OF DNA FRAGMENTS BY THE HYDROXYBENZOTRIAZOLE PHOSPHOTRIESTER APPROACH
Marugg, J. E.,Tromp, M.,Jhurani, P.,Hoyng, C. F.,Marel, G. A. van der,Boom, J. H. van
, p. 73 - 78 (2007/10/02)
The application of the phosphorylating agent 2-chlorophenyl-o,o-bis--phosphate (HOBT-approach) for the introduction of 3'-5'-phosphotriester linkages between a properly 5'-protected (4,4'-dimethoxytrityl) deoxy-nucleoside and a deoxy-nucleoside having free OH groups is described.In this way, all sixteen partially-protected DNA dimers having a free 3'-OH group have been synthesized.The dimers thus obtained have been applied, using the HOBT-approach, to the synthesis of the DNA fragments d-CGCGCG, d-CGTACG and d-ATATTAATAAC.
