93966-65-5Relevant academic research and scientific papers
SELF-COMPLEMENTARY TETRADEOXYRIBONUCLEOSIDE TRIPHOSPHATES. CONVENIENT CHEMICAL PREPARATION AND SPECTROSCOPIC STUDIES IN SOLUTION
Matsuzaki, Jun-ichi,Hotoda, Hitoshi,Sekine, Mitsuo,Hata, Tsujiaki,Higuchi, Shigesada,et al.
, p. 501 - 514 (2007/10/02)
Eight kinds of self-complementary tetradeoxyribonucleoside triphosphates were prepared by a simplified method which enabled us to omit purification of synthetic intermediates and provided the tetramers very rapidly and conveniently.The tetramers were characterized by enzyme assay and their conformations were studied by the use of UV and CD spectroscopic methods under various conditions.The detailed analysis of the CD spectra suggested that conformation of the tetramer duplexes was dependent on the sequence.
Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques
Seliger, Hartmut,Bach, Trung-Chinh,Siewert, Gerhard,Boidol, Werner,Toepert, Michael,et al.
, p. 835 - 853 (2007/10/02)
For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.
