950846-89-6

Usage

Uses

Used in Biological Research and Imaging Applications:
2-(2-Aminoethyl) Rhodamine B amide is used as a fluorescent dye for labeling and visualizing specific targets in cells and tissues. Its conjugation to proteins allows for the tracking and analysis of cellular processes and structures.
Used in Diagnostic Assays:
2-(2-Aminoethyl) Rhodamine B amide is used as a probe in the development of diagnostic assays, enabling the detection and quantification of specific biomolecules or cellular events.
Used in Studying Cellular Function and Intracellular Trafficking:
2-(2-Aminoethyl) Rhodamine B amide is used as a fluorescent probe to investigate cellular function and intracellular trafficking, providing insights into the dynamics and mechanisms of cellular processes.

Upstream product

• 509-34-2 rhodamine B 
• 107-15-3 ethylenediamine 
• 69319-23-9 rhodamine B 
• 81-88-9 rhodamine B 
• 1118784-85-2 rhodamine B 
• 111-40-0 3-azapentane-1,5-diamine 
• 1118426-88-2 rhodamine B hydrochloride 
• 4344-42-7 rhodamine B 

Downstream Products

• 1236996-19-2 C₄₄H₅₀B₂N₄O₆ 
• 1236996-20-5 C₄₄H₄₈N₄O₂ 
• 1375855-46-1 2-(benzyl(2-hydroxyethyl)amino)-N-(2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)ethyl)acetamide 
• 1415914-70-3 C₃₇H₄₀N₄O₃ 
• 1449506-22-2 C₃₉H₄₂N₄O₃ 
• 1428634-13-2 C₄₇H₄₇N₅O₅ 
• 1574486-45-5 Rhodamine B 

Relevant academic research and scientific papers

Rapid detection of mercury (II) ions and water content by a new rhodamine B-based fluorescent chemosensor

A rhodamine B-based sensor (RS) was designed and synthesized by a combination of the spirolacton rhodamine B (fluorophore) and multidentate chelates (ionophore) with high affinity towards Hg2+. In the presence of Hg2+, the resulting red-orange fluorescence (under UV light) and naked eye red color of RS are supposed to be used for quantitative and qualitative measurement of Hg2+. Further fluorescent titration and analysis demonstrate that RS can selectively detect Hg2+ within 1 s with a low limit of detection (LOD) of 16 nM in acetonitrile media, meanwhile, the association constant (Ka) was calculated to be 0.32 × 105 M?1. More importantly, the resultant complex (RSHg) of RS and Hg2+ has also been successfully applied to detect limited water content in acetonitrile solution.

A rhodamine based fluorescent probe for Hg2+ and its application to cellular imaging

A new rhodamine-based fluorescent probe (Rh-F) for detection of Hg 2+ ions was synthesized, which could bind Hg2+ in aqueous ethanol (7:3, v/v) at pH 7.0 with detectable change in color and fluorescence. The response is based on a ring opening reaction and formation of a 1:1 complex, while ring-opening process of spirolactam enables large fluorescent enhancement and colorimetric change upon the addition of Hg2+. The response is reversible and linear in the range between 200 nM and 1000 nM, with a detection limit of 4.2 nM. Selectivity and competition experiments with various other metal ion revealed that Rh-F possesses highly selective fluorescent response to Hg2+. Furthermore, the probe was successfully applied to fluorescent imaging of Hg2+ in L-929 cells confirm that Rh-F can be used as a fluorescent probe for monitoring Hg2+ in living cells.

A new Rhodamine B-based 'on-off' chemical sensor with high selectivity and sensitivity toward Fe3+ and its imaging in living cells

A new fluorescent chemosensor based on a Rhodamine B and pyrrole conjugate (RBPY) has been designed and synthesized. UV-vis absorption and fluorescence spectroscopic studies show that RBPY exhibits a high selectivity and sensitivity toward Fe3+ among many other metal cations in a MeOH/H2O solution (3:2, v/v, pH 7.10, HEPES buffer, 0.1 mM) by forming a 1:1 complex with Fe3+. Furthermore, results reveal that the formation of the RBPY-Fe3+ complex is fully reversible in the presence of sulfide anions and could also be used as an efficient sensor for S2-. Importantly, fluorescence microscopy experiments further demonstrated that RBPY can be utilized as a fluorescent probe for the detection of Fe3+ in human liver (L-02) cells.

Colorimetric and fluorescent nanofibrous film as a chemosensor for Hg 2+ in aqueous solution prepared by electrospinning and host-guest interaction

A fluorescent sensing film for Hg2+ ions was fabricated by host-guest interaction and electrospinning. When the nanofibrous film was put into a solution of Hg2+ ions, it gave rise to orange fluorescence, causing a clear color change from white to pink-red.

Insights into the phenomenon of acquisition and accumulation of Fe3+ in Hygrophila spinosa through fluorimetry and fluorescence images

Rhodamine functionalized fluorescent probe IP has been synthesized to investigate the phenomenon of Fe3+ acquisition and accumulation in Hygrophila spinosa. H. spinosa is a tropical medicinal plant which is iron rich and consumed for the treatment of the patients suffering from anaemia. IP is capable of selectively binding Fe3+ by enhancing fluorescent intensity via “turn on” mechanism due to complex formation. Spectroscopic studies and microscopic tools helped in better understanding about the acquisition as well as the quantitative accumulation of Fe3+ in different parts of the plant.

A dual FRET based fluorescent probe as a multiple logic system

A new fluorescent probe with a unique sequential dual FRET process was designed and constructed. This probe showed distinguished responses towards Fe3+ and Hg2+, making it an ideal candidate for multiple logic operations at the molecular level.

Mechanically controlled FRET to achieve high-contrast fluorescence switching

Organic luminescent materials with the ability to reversibly switch the luminescence when subjected to external stimuli have attracted considerable interest in recent years. However, luminescent materials with mechanochromic and photochromic dual-responsi

Lanthanide(III) complexes of rhodamine-DO3A conjugates as agents for dual-modal imaging

Two novel dual-modal MRI/optical probes based on a rhodamine-DO3A conjugate have been prepared. The bis(aqua)gadolinium(III) complex Gd.L1 and mono(aqua)gadolinium(III) complex Gd.L2 behave as dual-modal imaging probes (r1 = 8.5 and 3.8 mM-1 s-1 for Gd.L1 and Gd.L2, respectively; λex = 560 nm and λem = 580 nm for both complexes). The rhodamine fragment is pH-sensitive, and upon lowering of the pH, an increase in fluorescence intensity is observed as the spirolactam ring opens to give the highly fluorescent form of the molecule. The ligands are bimodal when coordinated to Tb(III) ions, inducing fluorescence from both the lanthanide center and the rhodamine fluorophore, on two independent time frames. Confocal imaging experiments were carried out to establish the localization of Gd.L2 in HEK293 cells and primary mouse islet cells (～70% insulin-containing β cells). Colocalization with MitoTracker Green demonstrated Gd.L2's ability to distinguish between tumor and healthy cells, with compartmentalization believed to be in the mitochondria. Gd.L2 was also evaluated as an MRI probe for imaging of tumors in BALB/c nude mice bearing M21 xenografts. A 36.5% decrease in T1 within the tumor was observed 30 min post injection, showing that Gd.L2 is preferentially up taken in the tumor. Gd.L2 is the first small-molecule MR/fluorescent dual-modal imaging agent to display an off-on pH switch upon its preferential uptake within the more acidic microenvironment of tumor cells.

A new highly sensitive and selective fluorescence chemosensor for Cr 3+ based on rhodamine B and a 4,13-diaza-18-crown 6-ether conjugate

A rhodamine B-based sensor consisting of two rhodamine B moieties linked to the two amine sides of a 4,13-diaza-18-crown-6 ether was synthesized as a highly sensitive fluorescent probe for monitoring Cr3+ in a MeOH-H2O (3 : 2, v/v, pH 7.2) solution and in living cells.

Inkpen-printed reusable colorimetric sensors for the detection of Hg(ii)

An inkpen-based reversible biodegradable sensor (IRBS) has been developed for the detection of mercuric ions (Hg2+). The colour response can be tuned to allow detection with the naked eye based on Hg2+-induced opening of the rhodamine spirocycle, leading to an increase in fluorescence and other colour changes. After dipping in water, the colour change in the IRBS paper can be quantified by visual comparison with standard images of IRBS papers, allowing the concentration of Hg2+ to be determined over the concentration range 4.0 × 10-8 M to 1.0 × 10-6 M. Reusability has been established by repeatedly dipping and rinsing the paper in aqueous Hg2+ and EDTA solutions. This approach provides a sensitive and accurate method for the estimation of Hg2+ in environmental and biological applications. This journal is