98693-55-1Relevant academic research and scientific papers
Enzymatic Synthesis of 2-Deoxyribose 1-Phosphate and Ribose 1 Phosphate and Subsequent Preparation of Nucleosides
Kulikova, Irina V.,Drenichev, Mikhail S.,Solyev, Pavel N.,Alexeev, Cyril S.,Mikhailov, Sergey N.
, p. 6999 - 7004 (2019)
α-Ribose-1-phosphate (Rib-p) and 2-deoxy-α-ribose-1-phosphate (dRib-p) are key intermediates in nucleoside metabolism and are important starting compounds for the enzymatic synthesis of various modified nucleosides. To date, chemical and enzymatic methods allowed the preparation of these compounds in rather low yields (11–37 %). This prevents their widespread use for the enzymatic synthesis of biologically active and practically important nucleosides. Here we propose to use 7-methyl-2′-deoxyguanosine (7-Me-dGuo) and 7-methylguanosine (7-Me-Guo) for the preparation of dRib-p and Rib-p. In this paper, we present the effective preparation of Rib-p and dRib-p starting from readily prepared 7-methylguanosine derivatives via their irreversible enzymatic phosphorolysis in the presence of purine nucleoside phosphorylase. Rib-p and dRib-P are obtained in nearly quantitative yields (HPLC analysis) and 74–96 % yields after their isolation and purification, which is much higher than previously reported.
First stereoselective synthesis of 2-deoxy-alpha-D-ribosyl-1-phosphate: novel application of crystallization-induced asymmetric transformation.
Komatsu, Hironori,Awano, Hirokazu
, p. 5419 - 5421 (2007/10/03)
A first stereoselective synthesis of bis(cyclohexylamine) 2-deoxy-alpha-D-ribosyl-1-phosphate has been achieved. The synthesis features a key crystallization-induced asymmetric transformation (AT) to generate a desired alpha-anomer in 99% yield at a 98.8:1.2 ratio of alpha/beta.
