Reference

Detection of soluble peptidoglycan in urine after penicillin administration

Detection of soluble peptidoglycan in urine after penicillin administration. Park, Hydow; Zeiger, Allen R.; Schumacher, H. Ralph (Dep. Biochem., Thomas Jefferson Univ., Philadelphia, PA 19107, USA). Infect. Immun., 43(1), 139-42 (English) 1984. CODEN: INFIBR. ISSN: 0019-9567. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) A unique enzyme-linked immunosorbent assay was developed to detect sol. peptidoglycans in biol. fluids. It makes use of the similar affinities of vancomycin and purified rabbit antibodies to peptidoglycan precursor sequences found in sol.In this article, certain chemicals are used. One of their cas registry numbers is 132-98-9 peptidoglycans. This assay was used to detect as little as 500 pg sol. peptidoglycan/mL serum and 5 pg/mL of urine. Studies of normal individuals and Staphylococcus aureus-infected patients revealed only a few sera with detectable levels of sol. peptidoglycans. Studies of normal volunteers who were given a single oral dose of 250 mg penicillin VK showed that about half had detectable levels of sol. 132-98-9 is also in the experiment. peptidoglycans in their urine up to 6 h after ingestion. This suggests that sol. peptidoglycans can be released by indigenous bacteria in detectable amts. In 1 volunteer, a detectable level of sol. peptidoglycan in the urine at 6 h decreased to an undetectable level at 12 h. Such an ephemeral appearance of sol. peptidoglycan in the urine could account for the small no. of human sera that had detectable levels of sol. peptidoglycan. ..

Infrared spectroscopy of aqueous antibiotic solutions

Infrared spectroscopy of aqueous antibiotic solutions. Wong, James S.; Rein, Alan J.; Wilks, Donald; Wilks, Paul, Jr. (IBM Instrum., Inc., Danbury, CT 06810, USA). Appl. Spectrosc., 38(1), 32-5 (English) 1984. CODEN: APSPA4. ISSN: 0003-7028. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 64 The IR spectra of aq. antibiotic solns. were obtained by using a new Liq. Analyzer accessory in a Fourier-transform IR (FT-IR) spectrometer. Based on a cylindrical internal reflectance element, the accessory provides a sufficiently short (~0.015 mm) effective path length for IR spectroscopy of aq. solns. from 3200 to 800 cm-1. Spectra of aq. solns. of penicillin V K [132-98-9], Na oxacillin [1173-88-2], and Na methicillin [132-92-3] are shown. By measuring the absorbance of the b-lactam carbonyl band, FT-IR spectroscopy with the accessory is sensitivity to <0.1% by wt. of antibiotic both in aq. soln. 132-98-9 and 132-92-3 which are cas registry numbers of substances are two of reagents here. and in fermn. broth. The method may be useful for monitoring fermn. reactions and subsequent chem. modifications. .