Reference

Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography-densitometry

Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography-densitometry. I. Aesculus hippocastaneum L.Several substances are used for example 524-30-1 and 497-76-7 which are their cas registry numbers., Arctostaphyllos uva-ursi Spreng, Fraxinus excelsior L., Gentiana lutea L., Glycyrrhiza glabra L., Hamamelis virginiana L., Hypericum perforatum L., Olea europea L., Salix alba L. and Silybum marianum Gaertn. Vanhaelen, M.; Vanhaelen-Fastre, R. (Pharm. Inst., Free Univ. Brussels, Brussels B-1050, Belg.). J. Chromatogr., 281, 263-71 (English) 1983. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) TLC-densitometry was used to det. the biol. active or characteristic components of exts. of 10 medicinal plants. The plant, component, mobile phase, plate stationary phase if other than conventional silica gel 60 or silica gel 60F254, and wavelength for detection were: Aesculus hippocastaneum, aescin [6805-41-0], 1,2-dichloroethane-EtOH-MeOH-H2O (:) 530 nm (spraying with 1% vanillin and 5% H2SO4 in alc.); Arctostaphyllos uva-ursi, arbutin [497-76-7], EtOAc-MeOH-H2O (:13), 225 nm; Fraxinus excelsior, fraxin [524-30-1], EtOAc-EtCOMe-H2O-HCO2H (5:3:2:1), 365 nm fluorometry, excitation; Gentiana lutea, gentiopicrin [20831-76-9], 1,2-dichloroethane-MeOH-H2O (:1), 280 nm; Glycyrrhiza glabra, glyrrhizic acid [1405-86-3] (as NH4 salt), CH2Cl2-EtOH-MeOH-25% NH4OH (:), 258 nm; Hamamelis virginiana, gallic acid [149-91-7], CHCl3-EtOH-HCO2H (5:4:1), 300 nm; Hypericum perforatum hypericin [548-04-9] and pseudohypericin [55954-61-5], MeCN on RP-8 high-performance plates, 313 nm fluorometry, excitation; Olea europea, oleuropein [32619-42-4], CH2Cl2-MeOH-H2O (:1.5), 245 nm; Salix alba, salicum [138-52-3], CH2Cl2-MeOH-H2O (:1) on high-performance silica gel 60 plates, 270 nm; Silybum marianum, silybin [22888-70-6], PhMe-EtOAc-HCO2H (:5), 295 nm. Relative std. deviations were 3-3.5%, and recoveries were 95-99%. .

Change in salicin content during processing of wicker

Change in salicin content during processing of wicker. Zurakowski, Mieczyslaw; Pradzynski, Wlodzimierz (Inst. Chem. Technol. Drewna, Akad. Roln., Poznan, Pol.). Rocz. Akad. Roln. Poznaniu, 92, 105-11 (Polish) 1977. CODEN: RARPCF. DOCUMENT TYPE: Journal CA Section: 43 (Cellulose, Lignin, Paper, and Other Wood Products) The green twigs of Salix Americana contain max. 0.51% salicin (I) [138-52-3]; storage for 2 months decreases the concn. of I to 0.47%. The steeping of twigs or their heating in water at 100.degree. for 6 h reduces I concns. to 0.29 or 0.25%, resp. Heating in superheated water-steam mixt. at 140.degree. for 2 h destroys all I of wicker. The initial color of wicker twigs has no relation to the concns. of I.