Reference

Tylosin macrolide antibiotics from Streptomyces

Tylosin macrolide antibiotics from Streptomyces. Celmer, Walter D.; Cullen, Walter P.; Shibakawa, Riichiro; Tone, Junsuke (Pfizer Inc., USA). U.S. US 4411892 A 25 Oct 1983, 7 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: A61K031-71; C07H017-08; C12P019-62. NCL: 424181000. APPLICATION: US 82-374223 3 May 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Two macrolide antibiotics are produced by fermn. with Streptomyces albus ATCC 39012. Thus, a preculture was inoculated into a prodn. medium of cerelose 10, corn starch 20, yeast BYF 5.0, casein hydrolyzate 5.0, CoCl2 0.002, and CaCO3 1.0 g/L and incubated 12-30 h at 30° with shaking. The broth filtrate was extd. with the Me iso-Bu ketone. The ext. was worked up by repeated extn. between aq. and org. systems and chromatog. on silica gel to isolate 150 mg antibiotic complex from 2000 gal broth. I [88378-52-3], the major compd., was isolated from II [88378-53-4] on a reverse phase silica gel C18 column, eluting with MeOH-H2O-H2N(CH2)2OH (:0.5).Except for chemicals metioned above, 88378-53-4 and 88378-52-3 are also used. .

Detection of some antibiotics by thin-layer chromatography/flame ionization detector method

Detection of some antibiotics by thin-layer chromatography/flame ionization detector method. Saito, Tomio; Kaneshima, Hiroyasu; Okada, Michinori (Hokkaido Inst. Public Health, Sapporo 060, Japan). Hokkaidoritsu Eisei Kenkyushoho, (33), 131-3 (Japanese) 1983. CODEN: HOEKAN. ISSN: 0441-0793. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Detn. of 10 antibiotics was studied by thin-layer chromatog. using the flame ionization detector (TLC-FID). The antibiotics investigated were benzylpenicillin [61-33-6], chloramphenicol [56-75-7], spiramycin [8025-81-8], kitasamycin [1392-21-8], tylosin [1401-69-0], chlorotetracycline [57-62-5], oxytetracycline [79-57-2], dihydrostreptomycin [128-46-1], fradiomycin [1404-04-2], and kanamycin [8063-07-8]. These antibiotics were sepd. and detd. by a TLC-FID automated analyzer using a combination of 2 types of TLC rod and 4 types of developing solvent. Detection limits were 0. 56-75-7 and 57-62-5 which are cas registry numbers of substances are two of reagents here.2-0.5 mg. .