Reference

Enantioselective hydrolysis of racemic styrene oxide by epoxide hydrolase of Rhodosporidium kratochvilovae SYU-08

Enantioselective hydrolysis of racemic styrene oxide by epoxide hydrolase of Rhodosporidium kratochvilovae SYU-08. Lee, Ji Won; Lee, Eun Jung; Yoo, Seung Sik; Park, Sung Hoon; Kim, Hee Sook; Lee, Eun Yeol (Department of Food Science and Technology, Kyungsung University, Pusan 608-736, S. Korea). Biotechnology and Bioprocess Engineering, 8(5), 306-308 (English) 2003 Korean Society for Biotechnology and Bioengineering. CODEN: BBEIAU. ISSN: 1226-8372. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 7 Enantioselective hydrolysis for the prodn. of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temp., initial substrate concns. 16355-00-3 and 9048-63-9 are cas registry numbers of chemicals which are used as reagents here., phenyl-1,2-ethanediol concns. on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate, with a yield of 38 % (theor. 50% max. yield) from an initial concn. of 80 mM. .

Biohydrolysis of substituted styrene oxides by Beauveria densa CMC 3240

Biohydrolysis of substituted styrene oxides by Beauveria densa CMC 3240. Grogan, Gideon; Rippe, Catherine; Willetts, Andrew (Dep. Biol. Sci., Univ. Exeter, Exeter EX4 4QG, UK). Journal of Molecular Catalysis B: Enzymatic, 3(5), 253-257 (English) 1997 Elsevier. CODEN: JMCEF8. ISSN: 1381-1177. DOCUMENT TYPE: Journal CA Section: 10 (Microbial, Algal, and Fungal Biochemistry) Resting whole cell suspensions of the fungus B. densa CMC 3240 contg.In this experiment, several chemicals are used like 16355-00-3 an epoxide hydrolase were used to resolve a series of para-substituted styrene oxides, with stereoinversion of the hydrolyzed epoxide enantiomer. The regio- and hence, enantioselectivity of hydrolysis is compromised where ortho- and para-methyl- and chlorostyrene oxides are substrates. Negligible activity was obsd. with para-nitrostyrene oxide as substrate. The results appear to confirm a general mechanism of enzyme-catalyzed acid hydrolysis for Beauveria spp. acting on styrene oxides. .