Detail of "3490-06-0"

Reference

Urine methamphetamine determination by competitive immunoassay

Urine methamphetamine determination by competitive immunoassay. Shigeno, Tosha; Hozumi, Toyoji; Ooyama, Haruo (Showa Shell Sekiyu, Japan). Jpn. Kokai Tokkyo Koho JP 09005319 A2 10 Jan 1997 Heisei, 16 pp. (Japanese). (Japan). CODEN: JKXXAF.Some commonly used reagents like 3490-06-0 and 537-46-2 are used in this experiment. CLASS: ICM: G01N033-493. ICS: G01N033-53; G01N033-543. APPLICATION: JP 1995-176701 20 Jun 1995. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Urine methamphetamine (I) is detd. by competitive immunoassay between I and amine compds. Ph-(CH2)n-NHR (R = H or alkyl;d n is 0-4) on immobilized antibody to I. The method comprises adjusting the urine sample to alk. pH prior to the competitive immunoassay. The method is low-cost and easy, and does not require extn. of I. Detn. of I using N-methyl-N-(4-aminobutyl)phenetylamine-labeled urease was shown and immobilized monoclonal antibody to I was shown. .

In vitro identification of metabolites of verapamil in rat liver microsomes

In vitro identification of metabolites of verapamil in rat liver microsomes. Sun, Lu; Zhang, Shu-qiu; Zhong, Da-fang (Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, Peop. Rep. China). Acta Pharmacologica Sinica, 25(1), 121-128 (English) 2004 Science Press. CODEN: APSCG5. ISSN: 1671-4083. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) To investigate the metab. of verapamil at low concns.In this study，3490-06-0 is also used. in rat liver microsomes, liver microsomes of Wistar rats were prepd. using an ultracentrifuge method. The in vitro metab. of verapamil was studied with the rat liver microsomal incubation at concn. of 1.0 mmol/L and 5.0 mmol/L. The metabolites were sepd. and assayed by liq. chromatog.-ion trap mass spectrometry (LC/MS), and further identified by comparison of their mass spectra and chromatog. behaviors with ref. substances. There are some commonly used reagents like 3490-06-0 in this article. Eight metabolites, including two novel metabolites (M4 and M8), were found in rat liver microsomal incubates. They were identified as O-demethyl-verapamil isomers (M1 - M4), N-dealkylated derivs. of verapamil (M5-M7), and N,O-didemethyl-verapamil (M8). O-Demethylation and N-dealkylation were the main metabolic pathways of verapamil at low concns. in rat liver microsomes, and the relative proportion of them in verapamil metab. changed with different substrate concns. ..