Detail of "5545-17-5"

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Development and validation of an HPLC method with post-column derivatization for assay of N-acetylcysteine in plasma

Development and validation of an HPLC method with post-column derivatization for assay of N-acetylcysteine in plasma.Several substances are used for example 5545-17-5 which is its cas registry number. Vander Heyden, Y.; Mangelings, D.; Van Brempt, J.; Spapen, H. (Dept. of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit Brussel, Brussels B-1090, Belg.). Acta Chromatographica, 14, 149-164 (English) 2004 Silesian University, Institute of Chemistry. CODEN: ATCREU. ISSN: 1233-2356. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 1, 64 A quant. reversed-phase HPLC method has been developed that enables detn. of both low endogenous and high therapeutic concns. of N-acetylcysteine (NAC) in plasma. The compd. is detected fluorimetrically after derivatization with ortho-phthalaldehyde in the presence of a primary amine. Validation of the method revealed injection and method repeatability were good. The linear range was adequate and the limit of quantification was between 0.4 and 0.6 mm. Recovery of N-acetylcysteine from plasma samples was also acceptable. This method was applied to plasma samples from patients with a clin. septic shock who had received very high doses of N-acetylcysteine. Six samples were taken at different times after administration of N-acetylcysteine. The blood-concn. profiles obtained indicate the method is suitable for following the evolution of NAC in plasma under these conditions and can therefore be used for pharmacokinetic profiling. .