Reference

N-Acylcarnosines

N-Acylcarnosines. (Ajinomoto Co., Inc., Japan). Jpn. Kokai Tokkyo Koho JP 58135868 A2 12 Aug 1983 Showa, 5 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C07D233-64. APPLICATION: JP 82-17090 5 Feb 1982. DOCUMENT TYPE: Patent CA Section: 34 (Amino Acids, Peptides, and Proteins) N-Acylcarnosines R-b-Ala-His-OH (R = Ac, Bz) were prepd. by coupling histidine with R-b-Ala-OR1 [R1 = OSO3- M+ (M+ = tertiary amine or alkali metal cation), OCO2R2 (R2 = alkyl), OPOCl2]. Thus, adding 32 g SO3 in CH2Cl2 to 52.4 g Ac-b-Ala-OH and 40.4 g Et3N in CH2Cl2 at <10° and then stirring at room temp. for 10 min gave Ac-b-Ala-OSO3H.In this experiment, several chemicals are used like 56353-15-2 and 78137-74-3 Et3N in CH2Cl2, which was treated with 31 g histidine in aq. NaOH to give 38 g Ac-b-Ala-His-OH. .

High-performance liquid chromatographic determination of N-a-acetyl-L-carnosine in equine plasma

High-performance liquid chromatographic determination of N-a-acetyl-L-carnosine in equine plasma. Dunnett, Mark (Equine Sports Medicine Unit, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, UK). Journal of Chromatography, B: Biomedical Applications, 688(1), 150-154 (English) 1997 Elsevier. CODEN: JCBBEP. ISSN: 0378-4347. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) N-a-Acetyl-L-carnosine (NAcCAR) in perchloric acid exts. of equine plasma was assayed by HPLC on a 3 mm Hypersil ODS (150′4.6 mm I.D.) column eluted with 5 mM phosphoric acid-1 mM triethylamine, pH 2.58. NAcCAR was isolated by solid-phase extn. on Isolute PRS (propylsulfonyl) columns. The HPLC mean retention time for NAcCAR was 5.9±0.2 min. 56353-15-2 which is the cas registry number is also used here. The recovery from plasma by solid-phase extn. was 93.9-99.7 and lower limit of detection in plasma was 0.18 mM. The normal NAcCAR concn. in equine plasma was 2.4±0.3 mM. The method was applied to the detn. of plasma concns. following oral and i.v. NAcCAR administration. .