Reference

Stevia sweetener analysis

Stevia sweetener analysis. (Shimadzu Corp., Japan). Jpn. Kokai Tokkyo Koho JP 58187857 A2 2 Nov 1983 Showa, 3 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: G01N031-08; G01N033-02. APPLICATION: JP 82-70026 26 Apr 1982. DOCUMENT TYPE: Patent CA Section: 17 (Food and Feed Chemistry) Sweeteners, i.e. stevioside [57817-89-7], rebaudisiode A [58543-16-1], B [58543-17-2], and C [63550-99-2], , and steviol bioside [41093-60-1], in Stevia prepns. are simultaneously detd. by high performance liq. chromatog. using acetonitrile-water extn. in the presence of phosphates, a phosphate-contg. solvent as mobil phase, and a silica amino deriv. as a stationary phase.

Potential sweetening agents of plant origin

Potential sweetening agents of plant origin. V. Improved high-performance liquid chromatographic separation of the Stevia rebaudiana sweet diterpene glycosides using linear gradient elution. Makapugay, H. C.; Nanayakkara, N. P. D.; Kinghorn, A. D. (Coll.Chemicals with cas numbers 63550-99-2 and 41093-60-1 also play role. Pharm., Univ. Illinois, Chicago, IL, USA). J. Chromatogr., 283, 390-5 (English) 1984. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Section cross-reference(s): 9 The 8 known diterpene glycosides (stevioside [57817-89-7], steviolbioside [41093-60-1], dulcoside A [64432-06-0], and rebaudioside A [58543-16-1], B [58543-17-2], C [63550-99-2], D [63279-13-0], and E [63279-14-1]) were sepd. from S. rebaudiana leaf CHCl3 and MeOH exts. by high-performance liq. chromatog. on a 25 cm ′ 0.4 mm Zorbax NH2 column with MeCN:H2O () pH 5 as eluting solvent and UV detection at 210 nm. No derivatization was required. Recoveries of stevioside, rebaudiosides A and C, and dulcoside A from spiked samples were 96, 89, 90, and 92%, resp. Steviolbioside and rebaudiosides B, D, and E were not detected in any samples due to their extns. into the solvents used. The method appears to be advantageous over alternative approaches, however, for the simultaneous detn. of stevioside, rebaudiosides A and C, and dulcoside A, which ranged from 4.6 to 8.1, 1.9 to 3.7, 0.85 to 2.1, and 0.41 to 0.66% of dried leaf, resp., for samples from 5 different geog. locations. .