Reference

Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography-densitometry

Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography-densitometry. I. Aesculus hippocastaneum L.Several substances are used for example 524-30-1 and 497-76-7 which are their cas registry numbers., Arctostaphyllos uva-ursi Spreng, Fraxinus excelsior L., Gentiana lutea L., Glycyrrhiza glabra L., Hamamelis virginiana L., Hypericum perforatum L., Olea europea L., Salix alba L. and Silybum marianum Gaertn. Vanhaelen, M.; Vanhaelen-Fastre, R. (Pharm. Inst., Free Univ. Brussels, Brussels B-1050, Belg.). J. Chromatogr., 281, 263-71 (English) 1983. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) TLC-densitometry was used to det. the biol. active or characteristic components of exts. of 10 medicinal plants. The plant, component, mobile phase, plate stationary phase if other than conventional silica gel 60 or silica gel 60F254, and wavelength for detection were: Aesculus hippocastaneum, aescin [6805-41-0], 1,2-dichloroethane-EtOH-MeOH-H2O (:) 530 nm (spraying with 1% vanillin and 5% H2SO4 in alc.); Arctostaphyllos uva-ursi, arbutin [497-76-7], EtOAc-MeOH-H2O (:13), 225 nm; Fraxinus excelsior, fraxin [524-30-1], EtOAc-EtCOMe-H2O-HCO2H (5:3:2:1), 365 nm fluorometry, excitation; Gentiana lutea, gentiopicrin [20831-76-9], 1,2-dichloroethane-MeOH-H2O (:1), 280 nm; Glycyrrhiza glabra, glyrrhizic acid [1405-86-3] (as NH4 salt), CH2Cl2-EtOH-MeOH-25% NH4OH (:), 258 nm; Hamamelis virginiana, gallic acid [149-91-7], CHCl3-EtOH-HCO2H (5:4:1), 300 nm; Hypericum perforatum hypericin [548-04-9] and pseudohypericin [55954-61-5], MeCN on RP-8 high-performance plates, 313 nm fluorometry, excitation; Olea europea, oleuropein [32619-42-4], CH2Cl2-MeOH-H2O (:1.5), 245 nm; Salix alba, salicum [138-52-3], CH2Cl2-MeOH-H2O (:1) on high-performance silica gel 60 plates, 270 nm; Silybum marianum, silybin [22888-70-6], PhMe-EtOAc-HCO2H (:5), 295 nm. Relative std. deviations were 3-3.5%, and recoveries were 95-99%. .

A comparison of the effect of benzopyrones and other drugs with anti-inflammatory properties on acid and neutral protease activity levels in various tissues after thermal injury

A comparison of the effect of benzopyrones and other drugs with anti-inflammatory properties on acid and neutral protease activity levels in various tissues after thermal injury. Piller, N. B. (Zool. Dep., Univ. Adelaide, Adelaide, Aust.). Br. J. 14769-73-4 and 9001-92-7 which are cas registry numbers are also used here. Exp. Pathol., 57(4), 411-18 (English) 1976. CODEN: BJEPA5. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) The benzopyrones enhanced acid proteinase [9001-92-7] activity levels in the edema fluid and the extracellular compartment of the skin. Levamisole (I) [14769-73-4] enhanced acid proteinase in the serum and extracellulr compartmet of the skin 6 h after thermal injury, whereas Reparil [6805-41-0] had the same effect at 24 h. Generally the benzopyrones had little or no effect on neutral proteinase levels, whereas I and Reparil caused their depression. The enzyme enhancing activity of these drugs correlated remarkably well with their edema reducing ability. The drugs which increased enzyme activity levels the most were the most effective in decreasing the edema. .