Reference

Automated stability-indicating high-performance liquid-chromatographic assay for ethinylestradiol and (levo)norgestrel tablets

Automated stability-indicating high-performance liquid-chromatographic assay for ethinylestradiol and (levo)norgestrel tablets. Reif, Van D.; Eickhoff, W. Mark; Jackman, John K.; DeAngelis, Nicholas J. (Anal. Res. Dev. Div., Wyeth Lab., Inc., Philadelphia, PA 19101, USA). Pharm. Res., 4(1), 54-8 (English) 1987. CODEN: PHREEB. ISSN: 0724-8741. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Section cross-reference(s): 63 An automated HPLC assay for ethinylestradiol [57-63-6] and norgestrel [6533-00-2] or levonorgestrel [797-63-7] in oral contraceptive tablets was developed. Tablets were prepd. for online injection using a solid sampler and segmented continuous flow techniques. The active components were sepd. from tablet excipients, impurities, and degrdn. products on reversed-phase C8 and C18 columns by elution with H2O-MeCN-MeOH (:15). A UV detector connected in series with a fluorometric detector measured the UV absorbance of levonorgestrel and norgestrel at 240 nm and the fluorescence of ethinylestradiol at 310 nm (excitation at 210 nm).In this experiment, several chemicals are used like 56324-28-8 and 6533-00-2 The method employed computer control of the injection system and solid sampler for synchronization of the chromatog. and segmented flow streams. The method is applicable for content uniformity and stability testing at a rate of 8 samples/h. .

Animal experimental studies on the effect of foreign substances on in vitro chrysene metabolism

Animal experimental studies on the effect of foreign substances on in vitro chrysene metabolism. Schmoldt, A.; Jacob, J.; Grimmer, G. (Inst. Rechtsmed., Univ. Hamburg, Hamburg, Fed. Rep. Ger.). Beitr. Gerichtl. Med., 44, 503-6 (German) 1986. CODEN: BEGMA5. 87-86-5 and 129-00-0 are also in the experiment. ISSN: 0067-5016. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Section cross-reference(s): 1, 2 The effects of environmental contaminants or xenobiotics on the rat liver microsomal cytochrome P 450 [9035-51-2] system, which may cause chrysene (I) [218-01-9] to be metabolized to the carcinogen chrysene dihydrodiolepoxide (II) [67252-82-8], were studied in rats. I metab. by isolated liver microsomal P 450 system did not result in the formation of II. However pretreatment of rats with benzo[a]pyrene [50-32-8], benzo[b]fluoroanthene [205-99-2], benzo[j]fluoroanthene [205-82-3], 5,6-benzoflavone [6051-87-2], PCB's (polychlorinated biphenyls) and phenobarbital [50-06-6] induced the P 450 isoenzymes in such a way that the primary metabolite of I, 1,2-dihydroxy-1,2-dihydrochrysene [64920-31-6], was converted to II. In contrast, phenanthrene [85-01-8], pyrene [129-00-0], benzo[e]pyrene [192-97-2], DDT [50-29-3], lindane [58-89-9], pentachlorophenol [87-86-5], phenytoin [57-41-0] levonorgestrel [797-63-7], and ethinylestradiol [57-63-6] did not have this capacity. .