1032678-42-4Relevant articles and documents
Small Molecule Recognition Triggers Secondary and Tertiary Interactions in DNA Folding and Hammerhead Ribozyme Catalysis
Mao, Jie,Desantis, Chris,Bong, Dennis
supporting information, p. 9815 - 9818 (2017/08/02)
We have identified tris(2-aminoethyl)amine (tren)-derived scaffolds with two (t2M) or four (t4M) melamine rings that can target oligo T/U domains in DNA/RNA. Unstructured T-rich DNAs cooperatively fold with the tren derivatives to form hairpin-like structures. Both t2M and t4M act as functional switches in a family of hammerhead ribozymes deactivated by stem or loop replacement with a U-rich sequence. Catalysis of bond scission in these hammerhead ribozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridging interactions between loop and stem. The simplicity of the t2M/t4M binding site enables programming of allostery in RNAs, recoding oligo-U domains as potential sites for secondary structure or tertiary contact. In combination with a facile and general method for installation of the t2M motif on primary amines, the method described herein streamlines design of synthetic allosteric riboswitches and small molecule-nucleic acid complexes.
A convenient synthesis of cyanine dyes: Reagents for the labeling of biomolecules
Kvach, Maksim V.,Ustinov, Alexey V.,Stepanova, Irina A.,Malakhov, Andrei D.,Skorobogatyi, Mikhail V.,Shmanai, Vadim V.,Korshun, Vladimir A.
experimental part, p. 2107 - 2117 (2009/04/05)
Four tetramethylindo(di)carbocyanine-derived carboxylic acids have been prepared using a modified one-pot procedure for the dye assembly. The acids were converted into oxysuccinimide esters and phosphoramidite reagents. The efficiency of the reagents in t
