- A direct radioimmunoassay for 5α-androstane-3α,17β-diol 17-glucuronide
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Synthesis of the 11α-hemiglutaryl derivative of 5α-androstane-3α,17β-diol 17-glucuronide (androstanediol-17G) starting from androsta-4,9(11)-diene-3,17-dione through a 10-step sequence and the preparation of its bovine serum albumin conjugate is described.By using this conjugate, antiserum was raised in rabbits which proved to be very specific for androstanediol-17G.A direct radioimmunoassay using a double antibody procedure is described for the measurement of androstanediol-17G from plasma without prior chromatography.Keywords: direct radioimmunoassay; androstanediol-17glucuronide; tritium as tracer
- Rao, Pemmaraju N.,Rodriguez, Angela M.,Moore, Perry H.,Cessac, James W.
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- SYNTHESIS OF A PRECURSOR FOR THE PREPARATION OF 9α,11α-TRITIATED 5α-ANDROSTANE-3α,17β-DIOL 17-GLUCURONIDE
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Starting from 11β-hydroxytestosterone, we achieved the synthesis of a strategic precursor, C-19(11) unsaturated 5α-androstane-3α,17β-diol 17-glucuronide (9a), for the preparation of 9α,11α-tritiated 5α-androstane-3α,17β-diol 17-glucuronide.We optimized the reaction conditions for catalytic reduction employing hydrogen and subsequent base hydrolysis followed by purification on Amberlite XAD-2 resin to obtain the saturated 5α-androstane-3α,17β-diol 17-glucuronide.
- Rao, P. Narasimha,Damodaran, K. M.
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- Microbiological Transformations Part 7. Microbiological Transformations of A-nor- and A-homo-5α-androstane derivatives with the fungus Cunninghamella elegans.
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The microbiological transformation of 17β-hydroxy-A-nor-5α-androstan-2-one, 17β-hydroxy-A-homo-5α-androstan-3-one and 5α-androstan-3,17-dione, by Cunninghamella elegans is dominated by 9α-hydroxylation whereas 17β-hydroxy-5α-androstan-3-one undergoes predominant 7α-monohydroxylation. 9α-Hydroxy-5α-androstane-3,17-dione and 9α-hydroxy-A-homo-5α-androstane-3,17-dione were synthesised by a sequence involving 11α-hydroxylation of the corresponding 3,17-diones by Aspergillus ochraceous.
- Crabb, Trevor A.,Ratcliffe, Norman M.
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p. 650 - 669
(2007/10/02)
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