Steroids p. 216 - 221 (1992)
Update date:2022-08-10
Topics:
Rao, Pemmaraju N.
Rodriguez, Angela M.
Moore, Perry H.
Cessac, James W.
Synthesis of the 11α-hemiglutaryl derivative of 5α-androstane-3α,17β-diol 17-glucuronide (androstanediol-17G) starting from androsta-4,9(11)-diene-3,17-dione through a 10-step sequence and the preparation of its bovine serum albumin conjugate is described.By using this conjugate, antiserum was raised in rabbits which proved to be very specific for androstanediol-17G.A direct radioimmunoassay using a double antibody procedure is described for the measurement of androstanediol-17G from plasma without prior chromatography.Keywords: direct radioimmunoassay; androstanediol-17glucuronide; tritium as tracer
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