- Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence
-
6-Oxoestriol-6-(O-carboxymethyl)oxime-aminobutylethyl-isoluminol conjugate was synthesized. This luminogenic estriol derivative enabled us to develop a solid phase immunoassay method for the determination of unconjugated estriol in serum of pregnant women by the measurement of the bound estriol-isoluminol conjugate upon oxidation with a hydrogen peroxide/microperoxidase system. The sensitivity of the assay was 700 pmol/l. Results obtained by radioimmunoassay and the described method showed good agreement (r = 0.95). The chemiluminescent method is applicable in the routine measurement of unconjugated estriol.
- Klingler,Haupt,Postel,Knuppen
-
-
Read Online
- Estriol hapten and antibody and application thereof in fast sensor method for uncojugated estriol detection
-
The invention relates to the technical field of biosensors and discloses an estriol hapten and antibody and the application thereof in a fast sensor method for uncojugated estriol detection. The structure of the hapten is scientifically designed, so that sensitivity and specificity of the antigen-antibody are improved; electro-deposition of a prussian blue-chitosan-nanogold composite membrane on the surface of an electrode is conducted, the estriol antigen is fixed to the surface of the membrane, a novel horse radish peroxidase labeled estriol antibody and a sample to be tested are added after an unbound site is sealed, the sample and a fixed antigen compete for a free enzyme labeled antibody, hydrogen peroxide is added after washing, a current signal is measured, a cyclic voltammetry estriol standard curve is obtained, the fast sensor method for uncojugated estriol detection is provided successfully, detection range is 0.001-100 ng/mL, and detection limit is 0.001 ng/mL. The method has the advantages of being easy to operate, high in sensitivity, quick, convenient and the like and can be well applied to ultra-sensitive and fast detection of estriol in the sample.
- -
-
Paragraph 0055
(2016/10/27)
-
- Specificity of antibodies to ovarian hormones in relation to the site of attachment of the steroid hapten to the peptide carrier
-
Estradiol-17β, estriol and progesterone were rendered antigenic by covalent attachment to bovine serum albumin through ring B or C of the steroid molecule. Coupling to lysine residues of the protein was effected via the 6-(O-carboxymethyl)-oximes of the estrogens and via the 6-(carboxymethylene) thioether or the 11α-hemisuccinate of progesterone, by use of the carbodiimide reagent. Antisera to the estradiol 6-conjugate raised in rabbits or goats distinguished estradiol-17β more efficiently from estrone, estradiol-17α and estriol than did antisera to estradiol-17β-hemisuccinate-BSA; neither serum reacted with non-phenolic steroids or non-steroidal estrogens. Antisera to estriol 6-conjugates showed minimal cross-reaction with estradiol-17β and estrone. Rabbit antisera against the progesterone 6-conjugate did not react with phenolic or C19-steroids, cr with corticosterone, and only feebly with 11-deoxycorticosterone and 20α-or 20β-dihydroprogesterone; significant crossreaction occurred with 17β-hydroxyprogesterone and 3β,17α-dihydroxypregn-5-en-20-one (3-4%), Δ5-pregnenolone (8-14%) and with 5β- and 5β-dihydro-progesterone (100%). Sera against the 11-conjugate were better able to recognize changes about the A-ring and failed to cross-react with 5α-dihydroprogesterone ( 3%). Both sera showed greater specificity than reported for antibodies to 20-conjugates of progesterone towards the D-ring and 17-sidechain and were similar in this respect to antibodies elicited by 3-conjugates described in the literature. It is concluded that the site of attachment of steroid haptens to the peptide carrier importantly affects the specificity of the antibodies produced.
- Lindner,Perel,Friedlander,Zeitlin
-
p. 357 - 375
(2007/10/14)
-
