2380-63-4

Basic information

• Product Name: 4-Aminopyrazolo[3,4-d]pyrimidine
• Synonyms: TIMTEC-BB SBB004205;1H-PYRAZOLO[3,4-D]PYRIMIDIN-4-YLAMINE;1H-PYRAZOLO[3,4-D]PYRIMIDIN-4-AMINE;4-AMINOPYRAZOLO[3,4-D]PYRIMIDINE;4-AMINO-1H-PYRAZOLO[3,4-D]PYRIMIDINE;ADENINE ANTIMETABOLITE;1H-Pyrazolo[3,4-d]pyrimidine, 4-amino-;4 APP
• CAS NO: 2380-63-4
• Molecular Formula: C₅H₅N₅
• Molecular Weight: 135.13
• EINECS: 219-174-6
• Product Categories: PYRIMIDINE;Pyridines, Pyrimidines, Purines and Pteredines;Heterocyclic Compounds;Heterocycles;Heterocycle-Pyrimidine series
• Mol File: 2380-63-4.mol

Chemical Properties

• Melting Point: >325 °C(lit.)
• Boiling Point: 238.81°C (rough estimate)
• Flash Point: 244.382 °C
• Appearance: Light tan solid
• Density: 1.3795 (rough estimate)
• Vapor Pressure: 0.00118mmHg at 25°C
• Refractive Index: 1.7000 (estimate)
• Storage Temp.: -20°C Freezer
• PKA: 12.12±0.20(Predicted)
• Water Solubility: Insoluble in water.
• BRN: 5824
• CAS DataBase Reference: 4-Aminopyrazolo[3,4-d]pyrimidine(CAS DataBase Reference)
• NIST Chemistry Reference: 4-Aminopyrazolo[3,4-d]pyrimidine(2380-63-4)
• EPA Substance Registry System: 4-Aminopyrazolo[3,4-d]pyrimidine(2380-63-4)

Safety Data

• Hazard Codes: T
• Statements: 25-36/37/38-23/24/25
• Safety Statements: 26-36/37/39-45-22-36
• RIDADR: UN 2811 6.1/PG 3
• WGK Germany: 3
• RTECS: UR0717000
• TSCA: Yes
• HazardClass: 6.1
• PackingGroup: III
• Hazardous Substances Data: 2380-63-4(Hazardous Substances Data)

Usage

Uses

Used in Pharmaceutical Industry:
4-Aminopyrazolo[3,4-d]pyrimidine is used as a therapeutic agent for the treatment of hypercholesterolemia in rats. It has been shown to decrease serum cholesterol markedly, which can be beneficial in managing and reducing the risk of cardiovascular diseases associated with high cholesterol levels.
Used in Chemical Research:
As a heterocyclic compound with a unique structure, 4-Aminopyrazolo[3,4-d]pyrimidine can be used as a building block or intermediate in the synthesis of more complex molecules for various applications in the chemical industry. Its reactivity and functional groups can be exploited to create novel compounds with specific properties and potential uses.
Used in Drug Design and Development:
The biological activity of 4-Aminopyrazolo[3,4-d]pyrimidine makes it a promising candidate for drug design and development. Researchers can use 4-Aminopyrazolo[3,4-d]pyrimidine as a starting point to develop new drugs targeting various diseases and conditions, particularly those related to cholesterol metabolism and cardiovascular health.

InChI:InChI=1/C5H5N5/c6-4-3-1-9-10-5(3)8-2-7-4/h1-2H,(H3,6,7,8,9,10)

Synthetic route

3-Amino-4-cyanopyrazole (16617-46-2) + formamide (77287-34-4, 77287-35-5, 60100-09-6) → 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
at 180℃;	100%
at 170℃; for 5h; Inert atmosphere;	94%
for 1h; Reflux;	93%

formamide (77287-34-4, 77287-35-5, 60100-09-6) + 3-amino-4-cyano-2H-pyrazole (16617-46-2) → 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
at 180℃; Inert atmosphere;	95%
at 180℃; for 2h; Microwave irradiation;	93%
at 180℃; for 2h; Microwave irradiation;	93%

3-Amino-4-cyanopyrazole (16617-46-2) → 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
In formamide	87%

3-Amino-4-cyanopyrazole (16617-46-2) → lH-pyrazolo[3,4-d]pyrimidin-4-ylamine + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
In formamide	A n/a B 87%

formamidine acetic acid (3473-63-0) + 3-amino-4-cyano-2H-pyrazole (16617-46-2) → 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
at 120℃; for 45h; Temperature; Concentration; Inert atmosphere;	84.1%
In ethanol for 5h; Reflux;	75%

N1,N1-dimethyl-N2-[4-cyanopyrazol-5-yl]formamidine (78972-81-3) → 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4)

Conditions	Yield
With ammonium hydroxide for 5h; Heating;	80%

4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 3-bromo-4-aminopyrazolo<3,4-d>pyrimidine (83255-86-1)

Conditions	Yield
With N-Bromosuccinimide In N,N-dimethyl-formamide at 60℃; for 3h;	100%
With N-Bromosuccinimide In N,N-dimethyl-formamide at 80℃; for 5h;	92%
With N-Bromosuccinimide In N,N-dimethyl-formamide at 60℃;	91%

4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 4-amino-3-iodo-1H-pyrazolo[3,4-d]pyrimidine (151266-23-8)

Conditions	Yield
With N-iodo-succinimide In N,N-dimethyl-formamide at 80℃;	100%
With N-iodo-succinimide In N,N-dimethyl-formamide at 80℃; for 5h;	98%
With N-iodo-succinimide In N,N-dimethyl-formamide for 12h;	97%

4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) + Hexafluoroacetone (684-16-2) → 2,2,4,4-tetrakis(trifluoromethyl)-2,8-dihydro<1,3,5>oxadiazino<3,4-c>pyrazolopyrimidine (125509-32-2)

Conditions	Yield
In N,N-dimethyl-formamide at 80℃; for 24h;	95%

di-tert-butyl dicarbonate (24424-99-5) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → C15H21N5O4

Conditions	Yield
With dmap In tetrahydrofuran at 20℃; Inert atmosphere;	90%

[(3aR,4S,6R, 6aR)-2,2-dimethyl-6-vinyl-4,5,6,6a-tetrahydro-3aH-cyclopenta[d][1,3]dioxol-4-yl] trifluoromethanesulfonate (1373333-65-3) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 1-((3aS,4R,6R,6aR)-2,2-dimethyl-6-vinyltetrahydro-3aHcyclopenta[d][1,3]dioxol-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine

Conditions	Yield
Stage #1: 4-Aminopyrazolo<3,4-d>pyrimidin With sodium hydride In N,N-dimethyl-formamide; mineral oil at 0℃; for 0.5h; Mitsunobu Displacement; Stage #2: [(3aR,4S,6R, 6aR)-2,2-dimethyl-6-vinyl-4,5,6,6a-tetrahydro-3aH-cyclopenta[d][1,3]dioxol-4-yl] trifluoromethanesulfonate In dichloromethane; N,N-dimethyl-formamide; mineral oil at 20℃; for 16h; Mitsunobu Displacement;	88%

N-iodo-succinimide (516-12-1) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 4-amino-3-iodo-1H-pyrazolo[3,4-d]pyrimidine (151266-23-8)

Conditions	Yield
In N,N-dimethyl-formamide at 80℃; for 12h;	86%
In N,N-dimethyl-formamide at 80℃; Concentration; Solvent;

4-phenoxyiodobenzene (2974-94-9) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (330786-24-8)

Conditions	Yield
With potassium tert-butylate In dimethyl sulfoxide at 120℃; for 24h; Inert atmosphere;	82%

Cyclopentyl bromide (137-43-9) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 1-cyclopentyl-1H-pyrazolo[3,4-d]pyrimidin-4-ylamine (21254-14-8)

Conditions	Yield
With caesium carbonate In N,N-dimethyl-formamide at 110℃;	80%

1-O-acetyl-2,3,5-tri-O-benzoyl-β-D-ribofuranose (6974-32-9) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → (2R,3R,4R,5R)-2-(4-amino-1H-pyrazolo[3,4-d]pyrimidin-1-yl)-5-((benzoyloxy)methyl)tetrahydrofuran-3,4-diyl dibenzoate (854020-39-6)

Conditions	Yield
With boron trifluoride diethyl etherate In nitromethane for 2h; Heating;	79%
With boron trifluoride diethyl etherate In nitromethane for 1.5h; Reflux;	59%
With boron trifluoride diethyl etherate In nitromethane Reflux;	42%

C16H18F4O5S + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 1-((3aS,4R,6S,6aR)-6-(4-fluorobenzyl)-2,2-dimethyltetrahydro-4H-cyclopenta[d][1,3]dioxol-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine

Conditions	Yield
Stage #1: 4-Aminopyrazolo<3,4-d>pyrimidin With sodium hydride In N,N-dimethyl-formamide; mineral oil at 0℃; for 0.5h; Stage #2: C16H18F4O5S In N,N-dimethyl-formamide; mineral oil at 20℃; for 2h;	78%

2'-Deoxyguanosine (961-07-9) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 4-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1H-pyrazolo[3,4-d]pyrimidine (17318-21-7)

Conditions	Yield
With purine nucleoside phosphorylase at 50℃; pH=7; aq. phosphate buffer; Enzymatic reaction;	77%

copper (II) carbonate hydroxide + (carboxymethyl(4-methylbenzyl)amino)acetic acid (166826-77-3) + water (7732-18-5) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → [Cu2(N-(p-methylbenzyl)iminodiacetate)2(μ2-N1,N8-4-aminopyrazolo[3,4-d]pyrimidine)(water)2]*4water

Conditions	Yield
Stage #1: copper (II) carbonate hydroxide; (carboxymethyl(4-methylbenzyl)amino)acetic acid In water at 50℃; Stage #2: water; 4-Aminopyrazolo<3,4-d>pyrimidin In isopropyl alcohol for 1h;	75%

1-(tert-Butoxycarbonyl)-3-(methanesulfonyloxy)piperidine (129888-60-4) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → tert-butyl 3-(4-amino-1H-pyrazolo[3,4-d]pyrimidin-1-yl)piperidine-1-carboxylate (1374250-98-2)

Conditions	Yield
With potassium carbonate In N,N-dimethyl-formamide at 100℃;	72%
Stage #1: 4-Aminopyrazolo<3,4-d>pyrimidin With sodium hydride In N,N-dimethyl-formamide; mineral oil at 20℃; for 0.5h; Stage #2: 1-(tert-Butoxycarbonyl)-3-(methanesulfonyloxy)piperidine In N,N-dimethyl-formamide; mineral oil at 100℃; for 16h;	50%

[1,3]-dioxolan-2-one (96-49-1) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 9-(2'-hydroxyethyl)adenine (100524-24-1)

Conditions	Yield
With sodium hydroxide In N,N-dimethyl-formamide for 2h; Heating;	71%

copper (II) carbonate hydroxide + water (7732-18-5) + (carboxymethyl(4-fluorobenzyl)amino)acetic acid (1813-23-6) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → [Cu4(N-(p-fluorobenzyl)iminodiacetate)4(μ2-N8,N9-4-aminopyrazolo[3,4-d]pyrimidine)2(water)]*water

Conditions	Yield
Stage #1: copper (II) carbonate hydroxide; (carboxymethyl(4-fluorobenzyl)amino)acetic acid In water at 50℃; Stage #2: water; 4-Aminopyrazolo<3,4-d>pyrimidin In isopropyl alcohol for 1h;	65%

1-azido-2,3-epoxypropane (80044-09-3) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 1-(4-Amino-pyrazolo[3,4-d]pyrimidin-1-yl)-3-azido-propan-2-ol

Conditions	Yield
With potassium carbonate In N,N-dimethyl-formamide at 80℃; for 8h;	64%

4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) + phenylboronic acid (98-80-6) → N-phenyl-1H-pyrazolo[3,4-d]pyrimidin-4-amine (99973-41-8)

Conditions	Yield
With [2,2]bipyridinyl; copper diacetate; caesium carbonate In N,N-dimethyl-formamide at 60℃; for 12h; Chan-Lam Coupling;	62%

1-methyl-piperazine (109-01-3) + formaldehyd (50-00-0) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 1-(N-methylpiperazinomethyl)-4-(N-methylpiperazinomethylamino)pyrazolo<3,4-d>pyrimidine (83255-90-7)

Conditions	Yield
In water	60%

2'-deoxyuridine (951-78-0) + 4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) → 4-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1H-pyrazolo[3,4-d]pyrimidine (17318-21-7)

Conditions	Yield
With purine nucleoside phosphorylase In dimethyl sulfoxide at 50℃; for 72h; pH=7; aq. phosphate buffer; Enzymatic reaction;	60%
at 37℃; for 16h; alginate gel-entrapped cells of auxotrophic thymine-dependent strain of E. coli, ammonium acetate buffer pH 5.7;

4-Aminopyrazolo<3,4-d>pyrimidin (2380-63-4) + benzyl alcohol (100-51-6) → N-benzyl-1H-pyrazolo[3,4-d]pyrimidin-4-amine (58360-86-4)

Conditions	Yield
With samarium diiodide; potassium tert-butylate In tetrahydrofuran; toluene at 140℃; for 1.5h; Microwave irradiation; Inert atmosphere;	59%

Upstream product

• 3473-63-0 formamidine acetic acid 
• 16617-46-2 3-amino-4-cyano-2H-pyrazole 
• 50-00-0 formaldehyd 
• 16617-46-2 3-Amino-4-cyanopyrazole 
• 315-30-0 Allopurinol 
• 77287-34-4 formamide 
• 3258-05-7 4-amino-1-(β-D-ribofuranosyl)-1H-pyrazolo[3,4-d]pyrimidine 
• 78972-81-3 N¹,N¹-dimethyl-N²-[4-cyanopyrazol-5-yl]formamidine 
• 5399-92-8 4-chloro-1H-pyrazolo[3,4-d]pyrimidine 

Downstream Products

• 1414357-57-5 (R)-tert-butyl (5-(2-((4-amino-3-(2-fluoro-4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)pyrrolidin-1-yl)-4-cyano-2-methyl-5-oxopent-3-en-2-yl)carbamate 
• 1414358-14-7 4-amino-N-[(1R)-2-[4-amino-3-(2-fluoro-4-phenoxy-phenyl)pyrazolo[3,4-d]pyrimidin-1-yl]-1-methyl-ethyl]-2-cyano-4-methyl-pent-2-enamide hydrochloride 
• 1419223-02-1 (R)-tert-butyl 3-(4-amino-3-(4-(4-(trifluoromethyl)pyridin-2-ylcarbamoyl)phenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)piperidine-1-carboxylate 
• 1414356-40-3 3-[(2R)-2-([4-amino-3-[4-(2,3-difluorophenoxy)phenyl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl]methyl)pyrrolidin-1-yl]-3-oxopropanenitrile 
• 1414353-20-0 (R)-2-(2-((4-amino-3-(4-(2,3-difluorophenoxy)phenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)pyrrolidine-1-carbonyl)-3-cyclopropylacrylonitrile 
• 1414356-29-8 3-[(2R)-2-([4-amino-3-[4-(2,6-difluorophenoxy)phenyl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl]methyl)pyrrolidin-1-yl]-3-oxopropanenitrile 
• 1414353-22-2 (R)-2-(2-((4-amino-3-(4-(2,6-difluorophenoxy)phenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)pyrrolidine-1-carbonyl)-3-cyclopropylacrylonitrile 
• 1414358-19-2 tert-butyl N-[(2S)-1-[4-amino-3-(2-fluoro-4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]propan-2-yl]carbamate 
• 1414358-11-4 tert-butyl N-[(1R)-2-[4-amino-3-(2-fluoro-4-phenoxy-phenyl)pyrazolo[3,4-d]pyrimidin-1-yl]-1-methyl-ethyl]carbamate 
• 1414357-70-2 (R)-3-(4-(2,3-difluorophenoxy)-2-fluorophenyl)-1-(piperidin-3-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 
• 1414357-67-7 (R)-tert-butyl 3-(4-amino-3-(4-(2,3-difluorophenoxy)-2-fluorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)piperidine-1-carboxylate 
• 1414356-90-3 3-[(2R)-2-([4-amino-3-[4-(2-fluorophenoxy)phenyl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl]methyl)pyrrolidin-1-yl]-3-oxopropanenitrile 
• 1414356-89-0 3-[4-(2-fluorophenoxy)phenyl]-1-((2R)-pyrrolidin-2-ylmethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 
• 1414353-33-5 (R)-2-(2-((4-amino-3-(4-(2-fluorophenoxy)phenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)pyrrolidine-1-carbonyl)-3-cyclopropyl acrylonitrile 

Relevant articles and documents

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