5112
J.-Z. Liu et al. / European Journal of Medicinal Chemistry 45 (2010) 5108e5112
5.21 (d, 1H, J ¼ 8.1 Hz, CH), 4.18e4.14 (m, 2H, NCH2), 3.96e3.70 (m,
4H, 2 OCH2), 3.04e3.02(m, 2H, CH2Ar),1.28e1.09 (m, 6H, 2CH3); 13
NMR (125 MHz, CDCl3, ppm) : 183.3, 170.8, 137.6, 137.1, 136.9,
129.4, 128.6, 128.5, 125.5, 64.0, 59.8, 54.3, 43.4, 38.3, 16.2; 31P NMR
(200 MHz, CDCl3, ppm) : 22.4; Anal. Calcd for C28H34N3O4PS (540):
Acknowledgments
C
d
The authors wish to thank the National Key Project for Basic
Research (2010CB126105) and the National Natural Science Foun-
dation of China (20872021) for the financial support.
d
C, 62.32; H, 6.35; N, 7.79%. Found: C, 62.53; H, 6.86; N 7.38%.
Supporting information
4.4. MTT assay against cancer cell proliferation
Supporting information associated with this article can be
All tested compounds were dissolved in DMSO (1e100 mM
solution) and subsequently diluted in the culture medium before
treatment of the cultured cells. Tested cells were plated in 96-well
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