Unraveling the origin of the major mutation induced by ultraviolet light, the C→T transition at dTpdC sites. A DNA building block for the cis-syn cyclobutane dimer of dTpdU

Article abstract of DOI:10.1016/S0040-4020(01)81791-9

One possible origin of the major mutation induced by ultraviolet light, the C→T transition at dTpdC sites, involves the replicative bypass of the cyclobutane dimer of dTpdC or its deamination product, the cis-syn dimer of dTpdU. In order to investigate the latter proposal, we have designed and synthesized a building block for the incorporation of the cis-syn dTpdU dimer into oligonucleotides by automated solid phase DNA synthesis technology.