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Organic & Biomolecular Chemistry
Page 6 of 7
ARTICLE
Org. Biol. Chem
concentration of 12i and 13a for 48 h. Then the percentage of
apoptosis cells was analyzed with flow cytometry by staining with PI
and annexin V-FITC. B. 12i and 13a induced cell cycle arrest of A549
cell line. Cells were stained with PI and analyzed by flow cytometry
after treatment with various concentration of 12i and 13a for 24 h.
Figure 6. A. Representive images of Compound 12i inhibiting A549
cell migration in the transwell assay different concentrations. B.
Statistical analysis result of cell migration of 12i, 13a and 1, ** p <
0.01, *** p < 0.001 versus the control group. C. and D. Compound
12i inhibits tumor metastasis via modulating the EMT and
upregulating the acetylation of tubulin. Cell lysates were extracted
after treating A549 with 12i or 1 for 48 h. Cell extracts were run on
SDS−polyacrylamide gels and probed using indicated antibodies.
DOI: 10.1039/C5OB02250A
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Acknowledgements
This work was partially supported by the grants from National
Basic Research Program of China (2012CB910404), National
Major Scientific and Technological Special Project for
"Significant New Drugs Development" (2013ZX09507001),
National Natural Science Foundation of China (81202407 and
81272463), The Science and Technology Commission of
Shanghai Municipality (15431902200) and Research Fund for
the Doctoral Program of Higher Education of China
(20120076120029).
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