Angewandte Chemie - International Edition p. 5852 - 5856 (2012)
Update date:2022-07-29
Topics:
Uttamapinant, Chayasith
Tangpeerachaikul, Anupong
Grecian, Scott
Clarke, Scott
Singh, Upinder
Slade, Peter
Gee, Kyle R.
Ting, Alice Y.
Bring your own copper: Copper-chelating azides undergo much faster click reactions (CuAAC) than nonchelating azides under a variety of biocompatible conditions. This kinetic enhancement allows site-specific protein labeling to be performed on the surface of living cells with only 10-40μM Cu I/CuII (see scheme). Detection sensitivity was also increased for CuAAC detection of alkyne-modified proteins and RNA. Copyright
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