Overproduction of CMP-sialic acid synthetase for organic synthesis

DOI: 10.1021/ja00036a044

Source and publish data:

Journal of the American Chemical Society p. 3901 - 3910 (1992)

Update date:2022-08-04

Topics:

Authors:

Liu, Jennifer Lin-Chun

Shen, Gwo-Jenn

Ichikawa, Yoshitaka

Rutan, James F.

Zapata, Gerardo

Vann, Willie F.

Wong, Chi-Huey

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Article abstract of DOI:10.1021/ja00036a044

The gene coding for Escherichia coli CMP-sialic acid synthetase (E.G. 2.7.7.43) was cloned and overexpressed in E. coli through a primer-directed polymerase chain reaction. Two plasmids were constructed to produce the native enzyme and a modified enzyme f

Full text of DOI:10.1021/ja00036a044

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