Journal of the American Chemical Society p. 3901 - 3910 (1992)
Update date:2022-08-04
Topics:
Liu, Jennifer Lin-Chun
Shen, Gwo-Jenn
Ichikawa, Yoshitaka
Rutan, James F.
Zapata, Gerardo
Vann, Willie F.
Wong, Chi-Huey
The gene coding for Escherichia coli CMP-sialic acid synthetase (E.G. 2.7.7.43) was cloned and overexpressed in E. coli through a primer-directed polymerase chain reaction. Two plasmids were constructed to produce the native enzyme and a modified enzyme f
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