The use of porcine liver (2->3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2->3)-β-D-Galp-(1->3)-D-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

Article abstract of DOI:10.1016/S0008-6215(00)90555-X

α-Neup5Ac-(2->3)-β-D-Galp-(1->3)-D-GlcpNAc (2) and, α-Neup5Ac-(2->3)-β-D-Galp-(1->3)-β-D-GlcpNAcOMBn were prepared on a large scale by the action of β-D-Galp-(1->3)-D-GalpNAc (2->3)-α-sialyltransferase (partially purified from porcine liver) on β-D-Galp-(1->3)-D-GlcpNAc and β-D-Galp-(1->3)-β-D-GlcpNAcOMBn, respectively.The trisaccharide 2 is the epitope of the tumor-associated carbohydrate antigen CA 50, highly expressed in human pancreatic adenocarcinoma.