576
Russ.Chem.Bull., Int.Ed., Vol. 62, No. 2, February, 2013
Vorob´ev et al.
I (arb. units)
of compound L, its dihydrochloride was obtained by the addition
of an excess of an alcohol solution of HCl to free base L. The
mixture was kept for 2 days, the solvent was evaporated, and the
700
1
residue was recrystallized from alcohol. M.p. 135 С. H NMR
(300 MHz, CDCl3), : 2.49 (m, 2 H, C(2)H2); 2.74 (m, 2 H,
N—CH2); 3.04 (s, 3 H, OCH3); 3.37 (s, 1 H, NH); 3.98 (dd, 2 H,
CH2Ph); 4.49 (m, 1 H, H(3)); 6.91, 7.48 (4H, AA´BB´, Ar); 7.36
(m, 3 H, Ph); 7.63 (m, 2 H, Ph); 9.41 (s, 3 H, NH3+). Found (%):
С, 57.02; Н, 6.62; Cl, 19.86; N, 7.61. C17H22N2O•2HCl. Calꢀ
culated (%): С, 56.82; H, 6.68; Cl, 19.51; N, 7.79.
600
This work was financially supported by the Presidium
of the Russian Academy of Sciences (Program R5 "Fundaꢀ
mental Sciences to Medicine"), the Division of Chemistry
and Materials Science of the Russian Academy of Sciencꢀ
es (Program No. 09 "Biomolecular and Medicine Chemꢀ
istry"), and the Russian Foundation for Basic Research
(Project No. 11ꢀ04ꢀ01245).
2
4 [Zn2+]/[L]
Fig. 2. Change in the fluorescence intensity ( = 300 nm) upon
coordination of diamine L with zinc ion.
d
300 nm (see Fig. 1, curve 2). The introduction of ZnCl2
into the solution increased the fluorescence intensity of
the ligand due to coordination with zinc ion (see Fig. 1,
curves 3—5; Fig. 2). Thus, diamine ligand L can be used
for zinc determination in an aqueous solution.
References
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(5×15 mL). The extract was dried over Na2SO4, and the solvent
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(80%). 1H NMR (300 MHz, CDCl3), : 1.89 (m, 2 H, C(2)H2);
2.67 (m, 2 H, N—CH2); 3.69 (s, 2 H, CH2Ph); 3.80 (s, 3 H,
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7.21—7.28 (m, 11 H, Ph + 2 HAr + NH + NH2). IR, /cm–1
3200, 3300 (NH2, NHR). To refine the elementary composition
:
Received October 29, 2012;
in revised form January 23, 2013