Incorporation Of 6-Thioguanosine Into Oligoribonucleotides

Article abstract of DOI:10.1016/00404-0399(50)0995O-

The preparation of N²-benzoyl-S⁶-cyanoethyl-6-thioguanosine and its incorporation into oligoribonucleotides, using standard phosphoramidite methods, is described. 6-Thioguanosine has been incorporated into the three guanosine sites of the central core of the hammerhead ribozyme resulting in 2- to 9-fold reductions in catalytic activity.