Synthesis of pyridinone ribonucleoside 3'-O-phosphoramidites and their incorporation into oligoribonucleotides

Article abstract of DOI:10.1016/0960-894X(96)00030-3

Protected pyridin-2- and pyridin-4-one ribonucleosides 3 and 9 were synthesized using a one-pot reaction of silylated bases with 1-O-acetyl-tri-O-benzoyl-β-D-ribofuranose (2) in the presence of CF₃SO₃SiMe₃. The nucleosides were converted in 4 steps into 3'-O-phosphoramidites 7 and 11 which were incorporated into hammerhead ribozyme substrates using solid-phase phosphoramidite chemistry.