Synthesis of immunocomponents for the measurement of lead (Pb) by fluorescence polarization immunoassay

DOI: 10.1016/S0040-4020(98)00060-X

Source and publish data:

Tetrahedron p. 3093 - 3112 (1998)

Update date:2022-08-04

Topics:

Authors:

Adamczyk, Maciej

Fishpaugh, Jeffrey R.

Heuser, Kevin J.

Ramp, John M.

Reddy, Rajarathnam E.

Wong, Martin

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Article abstract of DOI:10.1016/S0040-4020(98)00060-X

Three novel haptens (5b, 9b, 11b) were synthesized from porphobilinogen (2) and conjugated to bovine serum albumin to afford immunogens (6, 10, 12). Five fluorescent tracers (14, 17, 19, 29, 32) were prepared from porphobilinogen (2), or haptens (9b, 11b, 20b) in >98% purity. The immunogens (6, 10, 12) were utilized for generation of polyclonal antibodies and the best tracer-antibody combination was selected to construct the calibration curve for the measurement of lead.

Full text of DOI:10.1016/S0040-4020(98)00060-X

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