Tetrahedron p. 3093 - 3112 (1998)
Update date:2022-08-04
Topics:
Adamczyk, Maciej
Fishpaugh, Jeffrey R.
Heuser, Kevin J.
Ramp, John M.
Reddy, Rajarathnam E.
Wong, Martin
Three novel haptens (5b, 9b, 11b) were synthesized from porphobilinogen (2) and conjugated to bovine serum albumin to afford immunogens (6, 10, 12). Five fluorescent tracers (14, 17, 19, 29, 32) were prepared from porphobilinogen (2), or haptens (9b, 11b, 20b) in >98% purity. The immunogens (6, 10, 12) were utilized for generation of polyclonal antibodies and the best tracer-antibody combination was selected to construct the calibration curve for the measurement of lead.
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