Selective inhibition of human cathepsin S by 2,4,6-trisubstituted 1,3,5-triazine analogs

Article abstract of DOI:10.1016/j.bmc.2018.07.032

We report herein the synthesis and biological evaluation of a new series of 2,4,6-trisubstituted 1,3,5-triazines as reversible inhibitors of human cysteine cathepsins. The desired products bearing morpholine and N-Boc piperidine, respectively, were obtained in three to four steps from commercially available trichlorotriazine. Seventeen hitherto unknown compounds were evaluated in vitro against various cathepsins for their inhibitory properties. Among them, compound 7c (4-(morpholin-4-yl)-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazine-2-carbonitrile) was identified as the most potent and selective inhibitor of cathepsin S (Ki = 2 ± 0.3 nM). Also 7c impaired the autocatalytic maturation of procathepsin S. Molecular docking studies support that 7c bound within the active site of cathepsin S, by interacting with Gly23, Cys25 and Trp26 (S1 subsite), with Asn67, Gly69 and Phe70 (S2 subsite) and with Gln19 (S1′ pocket).

Full text of DOI:10.1016/j.bmc.2018.07.032

Accepted Manuscript
Selective Inhibition of Human Cathepsin S by 2,4,6-trisubstituted 1,3,5-triazine
analogs
Zahira Tber, Mylène Wartenberg, Jean-Eddy Jacques, Vincent Roy, Fabien
Lecaille, Dawid Warszycki, Andrzej J. Bojarski, Gilles Lalmanach, Luigi A.
Agrofoglio
PII:
S0968-0896(18)30675-8
https://doi.org/10.1016/j.bmc.2018.07.032
BMC 14469
DOI:
Reference:
Bioorganic & Medicinal Chemistry
To appear in:
Received Date:
Revised Date:
Accepted Date:
4 April 2018
10 July 2018
18 July 2018
Please cite this article as: Tber, Z., Wartenberg, M., Jacques, J-E., Roy, V., Lecaille, F., Warszycki, D., Bojarski,
A.J., Lalmanach, G., Agrofoglio, L.A., Selective Inhibition of Human Cathepsin S by 2,4,6-trisubstituted 1,3,5-
triazine analogs, Bioorganic & Medicinal Chemistry (2018), doi: https://doi.org/10.1016/j.bmc.2018.07.032
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers
we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and
review of the resulting proof before it is published in its final form. Please note that during the production process
errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Selective Inhibition of Human Cathepsin S by 2,4,6-trisubstituted
1,3,5-triazine analogs
Zahira Tbera, Mylène Wartenbergb, Jean-Eddy Jacquesa, Vincent Roya*, Fabien Lecailleb,
Dawid Warszyckic, Andrzej J. Bojarskic, Gilles Lalmanachb, Luigi A. Agrofoglioa
a Université d’Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France
b
INSERM, UMR 1100, Centre d’Etude des Pathologies Respiratoires, Université François Rabelais,
F-37032 Tours cedex, France
c
Medicinal Chemistry Department, Institute of Pharmacology, Polish Academy of Sciences, Kraków,
Poland
Corresponding author: Vincent Roy
*Email: vincent.roy@univ-orleans.fr
ABSTRACT
We report herein the synthesis and biological evaluation of a new series of 2,4,6-
trisubstituted 1,3,5-triazines as reversible inhibitors of human cysteine cathepsins. The
desired products bearing morpholine and N-Boc piperidine, respectively, were obtained in
three to four steps from commercially available trichlorotriazine. Seventeen hitherto unknown
compounds were evaluated in vitro against various cathepsins for their inhibitory properties.
Among them, compound 7c (4-(morpholin-4-yl)-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazine-
2-carbonitrile) was identified as the most potent and selective inhibitor of cathepsin S (Ki = 2
± 0.3 nM). Also 7c impaired the autocatalytic maturation of procathepsin S. Molecular
docking studies support that 7c bound within the active site of cathepsin S, by interacting
with Gly23, Cys25 and Trp26 (S1 subsite), with Asn67, Gly69 and Phe70 (S2 subsite) and
with Gln19 (S1’ pocket).
KEYWORDS: cathepsins, cysteine protease, protease inhibitors, 1,3,5-triazine analogs,
microwave irradiation
1. Introduction
Lysosomal cysteine cathepsins (Cat) have been involved in many physiological and
3
pathological processes such as alzheimer's disease,1 cancer,2 stroke and Ebola.4 Eleven
human cysteine cathepsins, i.e. cathepsins (B, C, F, H,…) have been identified.5,6 These
proteases share similar three-dimensional structures7, catalytic mechanism, and substrate
specificity.8,6 Cathepsins are primarily involved in cellular turnover and degradation of
endocytosed proteins. Moreover, a growing body of evidence supports that cathepsins play
specific functions during numerous pathophysiological events. For instance, Cat K, that is a
critical bone resorbing protease, corresponds to a clinically relevant target for osteoporosis
and bone metastasis treatment,9-12 while Cat S is an attractive target for drugs in autoimmune
diseases (e.g. rheumatoid arthritis), emphysema or neuropathic pain.13-14

Inhibitors of cysteine cathepsins frequently contain an electrophilic functional group,
capable of interacting with the nucleophilic cysteine residue located within the enzyme active
site. Such groups include aldehydes or vinyl sulfones. Since nitrile-based heterocycles have
been identified as potent and selective inhibitors of Cat K and Cat S, a special attention was
paid for this scaffold. 15
On our ongoing efforts to develop reversible cathepsin inhibitors, we have previously
reported the synthesis and the biological evaluation of 1,3,5-triazines substituted by a nitrile
function and a cyclohexylamine or a piperazine moiety.16 Among the synthesized
compounds, some cyclohexylamine derivatives exhibited a highly potent inhibitory effect (nM
17
range) against both Cat B/K/L/S (similarly to other reported cyclohexylamine derivatives)
more, we have shown by docking studies that the piperazine moiety of the best inhibitors fits
into the S1’ subsite and interacts with the side chain of Gln19 through a hydrogen bond.
Based on these results, we decided to substituted the cyclohexylamine and the piperazine
moiety by a morpholine and a N-piperidine as new pharmacophores, to conserve hydrogen
bond interactions with the S1’ pocket of cathepsins (Figure 1).
Figure 1: Modifications of 1,3,5-triazine scaffold.
Thus, we report herein the synthesis and biological evaluation of two series of hitherto
unknown 1,3,5-triazines substituted by either a nitrile, a morpholine or a piperidine moiety.
2. Results and Discussion
The synthesis of those new 2,4,6 trisubstituted triazines was based on our recently
reported procedure,16 from commercially available cyanuric chloride 1. (Scheme 1). The
mono-substituted products 2 and 3 with morpholine and N-Boc piperidine respectively, were
obtained in good yields, by nucleophic substitution of cyanuric chloride 1 in presence of 1.1
equiv of diisopropylethyl amine (DIPEA) in dichloromethane at 0-5°C for 2-4h. The second
nucleophilic substitution was carried out with appropriate amines in presence of 1.1 eq. of
DIPEA, in acetonitrile, under microwave activation (MW) during 60-90 minutes, to afford the
desired products 4a-d, 4g-j and 5a-h, in 59% to 89% yield. However under these conditions,
starting from compound 2 in presence of amine derivatives bearing electron withdrawing
group (NO2 at para or meta position for e and f respectively), products 4e and 4f were not
obtained. Thus, in order to obtain 4e and 4f, we decided to introduce first the two amines, e
and f, in presence of potassium carbonate (K2CO3) in acetone at 0-5°C for 1h to afford
products 6e-f in good yields. A second nucleophilic substitution with morpholine using the
same conditions led to 4e and 4f.

Scheme 1. Reagents and conditions: (a) DIPEA, CH2Cl2, 0-5°C, (b) DIPEA, CH3CN, 150°C, MW,
(c) KCN, DABCO, DMSO/H2O, RT, (d) NH2-R (e or f) K2CO3, acetone, 0-5°C, (e) TFA, CH2Cl2, RT.
The treatment of triazine 4a-j and 5a-d,f-h, with 1.1 equiv of potassium cyanide
(KCN) in the presence of 0.2 equiv of 1,4-diazabicyclo[2.2.2]octane (DABCO) in
dimethylsulfoxide DMSO, provided the corresponding nitrile products 7a-j and 8a-d,f-h in
good yield. Finally, the deprotection of Boc group of compounds 8a-d,f-h was achieved
under acidic condition and gave the desired piperidine series 9a-d,f-h.
2.1Inhibition of cysteine cathepsins
In the current study, the SAR of the triazines derivatives bearing nitrile group 7 and 9,
where the substitution of the phenyl amine moiety, by fluoro, nitro, methoxy or
trifluoromethoxy groups at meta or para position, were evaluated for their inhibitory
properties (IC50) towards human Cat B, K, L and S. The enzyme inhibition data of the
screening were expressed as IC50 values (50% inhibitory concentration) and summarized in
Table 1.

Table 1 : Inhibition of Cat B, K, L and S by compounds 7a-j, 9a-d and 9f-h
IC50 (µM)
Compounds Cat B
Cat K
Cat L
Cat S
7a
7b
7c
7d
7e
7f
7g
7h
7i
5 ± 0.7
2.3 ± 1
> 500
74 ± 9
60 ± 4
0.4 ± 0.2
0.2 ± 0.04
2 ± 0.4
4.6 ± 0.3
1.6 ± 0.1
N. I.
0.03 ± 0.003
0.08 ± 0.02
0.003 ± 0.0006
0.1 ± 0.03
0.02 ± 0.001
0.02 ± 0.001
0.02 ± 0.002
0.02 ± 0.001
0.8 ± 0.3
0.3 ± 0.2
0.01 ± 0.002
0.2 ± 0.1
0.2 ± 0.01
0.2 ± 0.02
0.5 ± 0.4
18 ± 3
0.1 ± 0.02
0.1 ± 0.02
0.03 ± 0.001
0.05 ± 0.01
0.7 ± 0.07
0.8 ± 0.5
0.6 ± 0.06
0.2 ± 0.1
0.1 ± 0.01
0.06 ± 0.006
0.03 ± 0.003
0.02 ± 0.001
0.1 ± 0.01
5.3 ± 0.3
0.2 ± 0.03
62 ± 11
1.3 ± 0.1
37 ± 2
11 ± 3
160 ± 50
1.9 ± 0.5
19 ± 1
32 ± 10
3.5 ± 0.3
10 ± 2
0.8 ± 0.2
1.8 ± 0.2
1.7 ± 0.2
4.3 ± 0.6
1.7 ± 0.4
7j
9.5 ± 4
9a
9b
9c
9d
9f
9g
9h
1.3 ± 0.08
0.6 ± 0.04
1.4 ± 0.2
0.5 ± 0.04
0.03 ± 0.002
0.4 ± 0.04
0.4 ± 0.02
CN
HN
N
N
R
N
N
N
H
H
0.01 ± 0.003
0.05 ± 0.008
0.4 ± 0.03
9a-d, 9f-h
Ki (µM)
Compounds Cat B
Cat K
0.7 ± 0.1
Cat L
N. I.
Cat S
0.002 ± 0.0003
7c
10
> 500
N. I.
0.003 ± 0.0008 0.005 ± 0.0005 0.03 ± 0.005
After titration by E-64, human cathepsins B, L, K and S (1 nM) were incubated in the presence of
triazine derivatives as described in details elsewhere (section 4.6), using Z-Phe-Arg-AMC
(benzyloxycarbonyl-phenylalanyl-arginine-4-methylcoumarin, 20 µM) as substrate for cathepsins B (Km
180 µM), L (Km = 0.8 µM) and K (Km = 9.7 µM), and Z-LR-AMC (20 µM) as substrate for Cat S (Km
=
=
23 µM). The enzyme inhibition data of the screening were expressed as IC50 values (average values).
N.I.: no inhibition. Furthermore, the inhibition constant (Ki) of the most potent inhibitor (compound 7c)
was determined using the Cheng-Prusoff equation.18 Ki values are expressed as mean ± SEM (n=3). A
newly described substrate-derived and reversible azaGly cathepsin inhibitor (compound 10) was used
as control. 19
Among the synthesized compounds, morpholine analogs (7a-j) exhibited an inhibition
for Cat S at the noticeable exception of 7i and 7j bearing (R)-or (S)-2-hydroxyl-2-
phenylethylamine. Conversely, they did not inhibit Cat B and Cat L (IC50 >1000 nM).
Moreover these morpholine derivatives demonstrated a weak inhibitory activity towards Cat
K, except for 7g and 7h having respectively a fluoro electron withdrawing group at para- or
meta- position of aromatic ring (IC50 ≤ 40 nM). If some compounds (7c, 7e, 7h), exhibiting
potent activity for CatS (IC50 ≤ 18 nM), only 7c showed high selectivity with an IC50 of 4 nM
for Cat S and no activity for Cat B, K and L (IC50 ≥1800 nM). The substitution of morpholine
for the compound 7c by piperidine moiety (compound 9c) resulted in loss of activity for Cat S
(IC50 >150 nM). The piperidine moiety is better tolerated by Cat K and Cat L and decrease
selectivity for Cat S inhibitory activity for compounds 9f and 9g.
2.2Docking studies

To elucidate the binding mode of 7c, molecular docking to the crystal structure of
cathepsin S from its complex with a potent inhibitor (PDB ID 3OVX, resolution 1.49 Å) was
performed. The protein structure was prepared in Protein Preparation Wizard,20 under default
settings (assigning bond orders, adding hydrogens, creation of disulfide bonds, deleting
waters beyond 5 from het groups, H-bond assignment and restrained minimization), whereas
three-dimensional structure, conformation and protonation states (at pH 7.4) of 7c were
generated by LigPrep (force field used OPLS2005, retention of specified chiralities and
22,23
generation of only one low energy ring conformation).21 Finally, Glide
was used for
covalent docking (Cys25 was the reactive residue, sampling nitrogen inversion, sampling ring
conformations with energy window equal to 2.5 kcal mol-1, penalizing nonplanar conformation
of amides, up to 100 steps during energy minimization and performing post-docking
optimization of each conformer to the enzyme model. Each pose was ranked according to
affinity score, and the highest scored pose was further analyzed. The binding mode of 7c
within the active site of Cat S was similar to that published previously,16 i.e. besides
covalently bound Cys25, the imine nitrogen formed a hydrogen bond with the side chain of
Gln19 (S1’pocket) and triazine part interacted mainly with S1 subsite residue Gly23.
However, compared to the previously published piperazine derivatives 24,25 more interactions
of 7c with the S2 site were observed; in particular, morpholine oxygen formed a hydrogen
bond with NH backbone of Asn67 (hydrogen bond distance: 2.29 Å). It caused that p-
trifluoromethoxyphenyl group was located in S2 site not as deeply as mentioned piperazine
derivatives but had contacts with Gly69, and was close enough to Phe70 to be stabilized by
π-π interactions.
Figure 2: Binding pose of compound 7c in the active site of Cat S (PDB ID: 3OVX). Residues
within a distance of 4Å from the inhibitor have been shown. Hydrogen bond and stacking interactions
between Cat S and 7c are in red and blue, respectively. Residues forming S1 subsite of Cat S are
labelled in red, S2 residues in blue and S1’ residues in green.
2.3Effect of 7c on the autocatalytic maturation of procathepsin S

In addition to neutrophile elastase and matrix metalloproteinases, Cat S has a highly
potent elastinolytic activity and readily participates to degradation and/or turnover of the
extracellular matrix (ECM). Interestingly, lung chronic inflammation and tissue remodelling
result partly from an imbalance between proteolytic enzymes and their inhibitors in favour of
proteolysis.26,27 Cat S was mostly found as its proform (zymogen) in human inflammatory
bronchoalveolar lavage fluids (BALFs).28 BALF procathepsins could be activated
autocatalytically, indicating that alveolar fluid procathepsins are a substantial proteolytic
reservoir of mature enzymes that may contribute to ECM breakdown. Thus, it could be
assumed that the control of the maturation of pro-Cat S in addition to mature Cat S inhibition
is a relevant approach. Here the in vitro activation of recombinant pro-Cat S was performed
as described in "Material & Methods" (section 4.7). After 5 hours of incubation, pro-Cat S was
processed into its active mature form (Figure 3, lane 2), as confirmed by measurement of its
enzymatic activity. Addition of E-64, a broad-spectrum irreversible inactivator of cysteine
cathepsins fully blocked the zymogen maturation (Figure 3, lane 3). On the other hand LHVS
(100 µM), a potent Cat S inhibitor, did not prevent the initial processing steps of pro-Cat S
(Figure 3, lane 4), since we observed the presence of an intermediate form of pro-Cat S
(grey arrow), due to autoproteolytic cleavages within the prosegment of Cat S.29
Nevertheless the conversion of zymogen to its mature active protease (black arrow) was
completely impaired.. Similarly to that observed for LHVS, a complete lack of active Cat S
was observed in the presence of the compound 7c (Figure 3, lane 5, 100 µM). It could be
noticed that processing of active Cat S was impaired by 7c in a dose-dependent manner
(Figure 3, lanes 5-9). Present data indicate that 7c is a highly potent inhibitor of Cat S but
also that 7c has the property of blocking the self-processing of its zymogen. Moreover the
dual potency of 7c supports the notion that the activity of Cat S can be controlled both
downstream (mature Cat S) and upstream (reservoir proform: pro-Cat S).
1
2
3
4
5
6
7
8
9
250
150
100
75
50
37
25
15
Figure 3: Effect of 7c on the autocatalytic maturation of procathepsin S. Recombinant
procathepsin S (pro-Cat S) was incubated as reported in the experimental section (section 4.7) at
26°C for 5 h in the absence or presence of 7c and then separated by 15% SDS-PAGE under reducing
conditions. Lane 1: pro-Cat S (control); lane 2: pro-Cat S (5 h incubation); lane 3: pro-Cat S with E-64
((L-3-carboxy-trans-2,3-epoxy-propionyl-leucylamide-(4-guanido)-butane: 100 µM) (5 h incubation);
lane 4: pro-Cat S with LHVS (morpholinourea-leucyl-homophenylalanine-vinyl-sulfone phenyl: 100 µM)
(5 h incubation); lanes 5-9: pro-Cat S with 7c (100 – 10 – 1 – 0.1 – 0.01 µM) (5 h incubation). Black

29
arrow corresponds to pro-Cat S, grey arrow to its intermediate form
active Cat S.
and white arrow to mature
3. Conclusion
We analyzed the ability of new 2,4,6-trisubstituted 1,3,5-triazine to inhibit various
human cathepsins. Six compounds 7c, 7g, 7h, 9a, 9f, 9g showed a potent activity for both
cathepsins S and K. However, 9g demonstrated a broad inhibitory potential for cathepsins K,
S and L. The substitution of triazine scaffold with p-trifluoromethoxyphenyl group (compound
7c) affords a highly selective and powerful inhibitor of Cat S (Ki = 2 ± 0.3 nM). Despite
noticeable achievements, current pharmacological inhibitors of cathepsins may still have
harmful side effects (see for example the recent example of odanacatib - D. Brömme et al.
30), pushing for the development of safer reversible inhibitors. According to Cat S is a
validated target for drugs in autoimmune diseases (e.g. rheumatoid arthritis) or neuropathic
pain,
molecule
7c
(4-(morpholin-4-yl)-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazine-2-
carbonitrile) represents a promising lead scaffold for further structural modifications.
4. Experimental section
4.1. Chemistry
General. Commercially available chemicals were of reagent grade and used as received.
The reactions were monitored by thin layer chromatography (TLC) analysis using silica gel
plates (Kieselgel 60F254, E. Merck). Compounds were visualized by UV irradiation. Column
1
13
chromatography was performed on Silica Gel 60 M (0.040-0.063 mm, E. Merck). H and C
NMR spectra were recorded at 250 nm (13C, 62.9 MHz) or at 400 nm (13C, 100.62 MHz).
Chemical shifts are given in parts per million using tetramethylsilane (TMS) as internal
standard. Coupling constants (J) are reported in Hertz (Hz) and multiplicities are reported as
s (singlet), d (doublet), t (triplet), q (quartet), bs (broad signal) and m (multiplet). High
Resolution Mass spectra were performed on a Bruker maxis mass spectrometer by the
“Fédération de Recherche” ICOA/CBM (FR2708) platform. Optical rotations were measured
at 20–25 °C with a PerkinElmer 341 polarimeter. Melting point was performed only after
recrystallization in adequate solvent. All reactions under microwave irradiation were
performed using the Microwave Biotage Initiator in 2–5 mL sealed tubes.
4.2. General procedure 1
The cyanuric chloride 1 (1 equiv) was added in dry CH2Cl2 and stirred for 15 min at 0°C.
Then a solution of morpholine or N-Boc piperidine (1 equiv) with DIPEA (1.1 equiv) in dry
CH2Cl2 was added slowly. The reaction mixture was stirred for 1-3h at the same temperature
(TLC control). After completion of the reaction, the mixture was washed with a solution of 1N
HCl (3 x 30 mL) then with water (2 x 40 mL) and extracted by CH2Cl2 or EtOAc. The organic
layer was dried over MgSO4, concentrated and purified by silica gel column chromatography
or by recrystallization in ethanol 95 to give the desired product.
4.2.1. 2,4-Dichloro-6-(morpholin-4-yl)-1,3,5-triazine (2)
The compound 2 was prepared from cyanuric chloride (2 g, 10.4 mmol, 1 equiv) and
morpholine (0,944 g, 10,84 mmol, 1 equiv) for 2h, following the general procedure 1. the
residue was purified by silica gel column chromatography (PE/EtAc 8:2) to give desired

product as a white solid (0,897 g,70 %); Mp: 159 °C ; 1H NMR (400 MHz, CDCl3):δ 3.91 (dd,
J = 5.7, 4.1 Hz, 4H), 3.77 (dd, J = 5.7, 4.1 Hz, 4H); 13C NMR (101 MHz, CDCl3):δ 170.7,
164.3, 66.6, 44.7 ; HRMS (ESI): m/z [M+H]+ calcd for C7H9Cl2N4O 235.0154, found 235.0148.
4.2.2. tert-Butyl 4-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]piperidine-1-carboxylate (3)
The compound 3 was prepared from 1 (2.71g, 13.56 mmol, 1 equiv) and 4-amino-1-Boc-
piperidine (2.5 g, 13.56 mmol, 1 equiv) for 4h, following the general procedure 1. The desired
product was obtained after recrystallization in ethanol as a white powder (4,55 g, 96 %); Mp:
173 °C; 1H NMR (400 MHz, CDCl3): δ 5.68 (bs, 1H) 4.08 (dt, J = 10.3, 5.5 Hz, 4H), 2.95 (t, J
= 12.2 Hz, 2H), 2.13 – 1.89 (m, 3H), 1.48 (s, 9H); 13C NMR (101 MHz, CDCl3): δ 171.1,
170.1, 165.1, 165.2, 154.6, 48.8, 48.7, 31.5, 28.4; HRMS (ESI): m/z [M+H]+ calcd for
C13H20Cl2N5O2 348.0995 found 348.0988.
4.2.3. 4,6-dichloro-N-(4-nitrophenyl)-1,3,5-triazin-2-amine (6e)
To cyanuric chloride 1 (0,6 g, 3,25 mmol, 1 equiv) and K2CO3 (0.494 g, 3.575 mmol, 1.1
equiv) dissolved in acetone (15 mL) stirring at 0-5° C was added a solution of 4-nitroaniline
(0.449 g, 3.25 mmol, 1 equiv) in acetone (15 mL). The reaction mixture was allowed to stir at
0-5 °C for 1 hour under nitrogen. The reaction mixture was poured over crushed ice. The
solid formed was collected by vacuum filtration and the resulting solid was dried overnight
under vacuum was obtained after recrystallization in ethanol 95 as a yellow solid (0.7 g, 76
1
%); Mp: 124°C; H NMR (250 MHz, CD3OD): δ 8.24 (d, J = 9.4 Hz, 2H), 7.89 (d, J = 9.4 Hz,
13
2H); C NMR (101 MHz, CD3OD):δ 164.3, 150.5, 148.2, 138.4, 129.5, 126.4, 118.8, 114.0.
HRMS (ESI): m/z [M+H]+ calcd for C9H6Cl2N5O2 285.9899 found 285.9892.
4.2.4. 4,6-dichloro-N-(3-nitrophenyl)-1,3,5-triazin-2-amine (6f)
Compound 6b was prepared from cyanuric chloride 1 (0.6 g, 3.25 mmol, 1 equiv) and 3-
nitroaniline stirred for 1h, following same procedure described for compound 6a. Compound
6b was obtained after recrystallization in ethanol as a yellow solid (0.84 g, 90 %); Mp: 273
°C; 1H NMR (250 MHz, CD3OD): δ 8.62 (t, J = 2.2 Hz, 1H), 8.03 – 7.91 (m, 2H), 7.59 (t, J =
13
8.4 Hz, 1H); C NMR (101 MHz, CD3OD):δ 164.5, 150.7, 148.5, 138.5, 129.6, 126.5, 118.9,
115.5; HRMS (ESI): m/z [M+H]+ calcd for C9H6Cl2N5O2 285.9899 found 285.9896.
4.3. General procedure 2
A microwave vial (2-5ml) was charged with 1 equiv of mon-subtituted triazine in acetonitrile.
Then 1.1 equiv of DIPEA and 1 equiv of aryl amine derivative were added. The vial was
sealed and heated under microwave irradiated between 10 to180 min at 150°C. After
completion of the reaction (monotored by TLC), the solvent was removed by vacuum
evaporation. The resultant mixture was dissolved in EtOAc or CH2Cl2, a solution of 2N HCl
was added then water. The layer organic was extracted whit EtOAc or CH2Cl2 and dried with
MgSO4, The product was purified by silica gel chromatography or recrisallized in ethanol 95.
4.3.1. 4-Chloro-N-(4-methoxyphenyl)-6-(morpholin-4-yl)-1,3,5-triazin-2-amine (4a)
The compound 4a was prepared from 2 (0.2 g, 0.85 mmol, 1 equiv) and p-anisidine (0.105 g,
0.85 mmol, 1 equiv) for 30 min following the general procedure 2. The desired product was
obtained after purification on chromatography column (PE/EtOAc: 8/2) as a yellow powder
1
(0.18 g, 66 %); Mp:162 °C; H NMR (400 MHz, CDCl3): δ 7.41 (d, J = 8.9 Hz, 2H), 6.91 (d, J
= 8.9 Hz, 2H), 3.84 (d, J = 10.5 Hz, 7H), 3.77 – 3.71 (m, 4H); 13C NMR (101 MHz, CDCl3): δ

156.4, 130.6, 122.6, 114.1, 66.6, 66.5, 55.5, 44.0. HRMS (ESI): m/z [M+H]+ calcd for
C14H17ClN5O2 322,0993 found 322.1066.
4.3.2. 4-Chloro-N-(3-methoxyphenyl)-6-(morpholin-4-yl)-1,3,5-triazin-2-amine (4b)
Compound 4b was prepared from 2 (0.2 g, 0.85 mmol, 1 equiv) and m-anisidine (0.105g,
0.85 mmol, 1 equiv) for 60 min following the general procedure 2. The desired product was
obtained after recrystallization in EtOAc/hexane (5:1) as a white solid (0.165 g, 59 %);Mp:
147 °C; 1H NMR (250 MHz, CDCl3) : δ 7.27 – 7.19 (m, 2H), 7.07 – 7.00 (m, 1H), 6.69 – 6.62
13
(m, 1H), 3.80 (d, J = 10.5 Hz, 7H), 3.75 – 3.67 (m, 4H); C NMR (63 MHz, CDCl3):δ 213.7,
212.4, 178.9, 160.1, 148.2, 138.9, 129.7, 112.7, 109.3, 106.5, 66.6, 55.3, 44.1 ; HRMS (ESI):
m/z [M+H]+ calcd for C14H17ClN5O2: 322.0993 found 322.1066.
4.3.3. 4-Chloro-6-morpholino-N-(4-(trifluoromethoxy)phenyl)-1,3,5-triazin-2-amine (4c)
Compound 4c was prepared from 2 (0.2 g, 0.85 mmol, 1 equiv) and p-trifluoromethoxyaniline
(c) (0,996 g, 0,85 mmol, 1 equiv) for 30 min, following the general procedure 2. The desired
product was obtained after recrystallization in ethanol as a white solid (0,22 g, 69 %) ; Mp:
1
198 °C; H NMR (250 MHz, DMSO-d6) :δ 7.71 (d, J = 8.6 Hz, 2H), 7.25 (d, J = 8.6 Hz, 2H),
3.84 (d, J = 4.7 Hz, 4H), 3.79 – 3.72 (m, 4H); 13C NMR (101 MHz, DMSO-d6):δ 164.6, 163.8,
145.1, 136.4, 121.7, 121.5, 66.6, 66.5, 44.1; HRMS (ESI): m/z [M+H]+ calcd for
C14H14ClF3N5O2 376.0789 found 376.078.
4.3.4. 4-Chloro-6-(morpholin-4-yl)-N-[3-(trifluoromethoxy)phenyl]-1,3,5-triazin-2-amine
(4d)
Compound 4d was prepared from 2 (0.2 g, 0.85 mmol, 1 equiv) and m-
trifluoromethoxyaniline (0.996 g, 0.85 mmol, 1 equiv) for 40 min, following the general
procedure 2. Compound 4d was obtained after purification on chromatography column
(PE/EtOAc: 8/2) as a white solid (0,21 g, 66 %); Mp: 147°C; 1H NMR (400 MHz,CDCl3):δ 7.79
(s, 1H), 7.36 (t, J = 8.2 Hz, 1H), 7.21 (d, J = 4.4 Hz, 1H), 6.99 – 6.94 (m, 1H), 3.90 – 3.77 (m,
8H); 13C NMR (101 MHz, CDCl3): δ 170.4, 169.5, 164.5, 163.7, 149.5, 139.2, 130.0, 121.7,
116.1, 113.0, 66.6, 66.4, 44.5, 44.2 ; HRMS (ESI): m/z [M+H]+ calcd for
C14H14ClF3N5O2 376.0789 found 376.079
4.3.5. 4-Chloro-6-morpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine (4e)
Compound 4e was prepared from 6e (0.2 g, 0.69 mmol, 1 equiv) and morpholine (0.0601g,
0.69 mmol, 1 equiv) for 30 min, following the general procedure 2. Compound 4e was
obtained after purification on column chromatography (EP/EtOAc: 8/2 to 6/4) as a yellow
1
solid (0.14 g, 60%); Mp: 264 °C; H NMR (400 MHz, DMSO-d6): δ 10.76 – 10.70 (m, 1H),
8.24 (d, J = 9.7 Hz, 2H), 8.00 – 7.83 (m, 2H), 3.93 – 3.60 (m, 8H); 13C NMR (101 MHz,
DMSO-d6) :δ 169.1, 164.0, 142.2, 125.3, 120.0, 66.2, 44.3; HRMS (ESI): m/z [M+H]+ calcd
for C13H14ClN6O3 337.0817 found 337.0809.
4.3.6. 4-Chloro-6-(morpholin-4-yl)-N-(3-nitrophenyl)-1,3,5-triazin-2-amine (4f)
Compound 4f was prepared from 6f (0.2 g, 0.69 mmol, 1 equiv) and morpholine (0.0601g,
0.69 mmol, 1 equiv) for 120 min, following the general procedure 2. Compound 4f was
obtained after purification on column chromatography.(PE/EtOAc: 8/2) as a yellow solid (0.16
1
g, 56 %); H NMR (400 MHz, DMSO-d6): δ 10.54 (s, 1H), 8.81 (s, 1H), 7.99 – 7.78 (m, 2H),
13
7.58 (t, J = 8.2 Hz, 1H), 3.88 – 3.57 (m, 8H); Mp: 244 °C; C NMR (101 MHz, DMSO-d6):δ

169.0, 164.4, 163.9, 148.3, 140.5, 130.4, 126.3, 117.7, 114.5, 66.2, 66.0, 44.4, 44.3; HRMS
(ESI): m/z [M+H]+ calcd for C13H14ClN6O3 337.0817 found 337.0806.
4.3.7. 4-chloro-N-(4-fluorophenyl)-6-morpholino-1,3,5-triazin-2-amine (4g)
Compound 4g was prepared from 2 (0.4 g, 1.97 mmol, 1 equiv) and p-fluoroaniline g
(0.133g, 1.197 mmol, 1 equiv) for 30 min, following the general procedure 2. Compound 4g
was obtained after recrystallization in ethanol then pentane as a brown solid (0.404 g, 77 %);
1
Mp: 185° C, H NMR (400 MHz, DMSO-d6):δ 7.79 (s, 1H), 7.36 (d, J = 8.4 Hz, 2H), 6.97 (d,
J = 8.4 Hz, 2H), 3.90 (d, J = 9.8 Hz, 2H), 3.77 (d, J = 4.4 Hz, 6H); 13C NMR (101 MHz,
DMSO-d6): δ 164.1, 163.6, 161.7, 152.2, 138.9, 130.2, 130.1, 115.7, 111.1, 110.8, 108.0,
107.7, 66.6, 44.2; 19F NMR (376 MHz, DMSO-d6):δ -117.37 (s); HRMS (ESI): m/z [M+H]+
calcd for C13H14ClFN5O 310.7294 found 310.0865.
4.3.8. 4-Chloro-N-(3-fluorophenyl)-6-morpholino-1,3,5-triazin-2-amine (4h)
Compound 4h was prepared from 2 (0.4 g, 1.197 mmol, 1 equiv) and m-fluoroaniline h
(0.133 g, 1.197 mmol, 1equiv) for 60 min following the general procedure 2. Compound 4h
was obtained after recrystallization in ethanol then pentane as a white solid (0.439 g, 84 %);
Mp: 166 °C; 1H NMR (250 MHz, DMSO-d6): δ 10.24 (s, 1H), 7.64 – 7.50 (m, 1H), 7.45 – 7.18
(m, 2H), 6.93 – 6.63 (m, 1H), 3.71-3.61 (d, J = m, 7 H), 3.26 (s, 1H); 13C NMR (101 MHz,
DMSO-d6): δ 164.1, 161.7, 152.2, 138.9, 130.2, 130.1, 115.8, 111.1, 110.8, 108.0, 107.7,
19
66.6, 44.2; F NMR (376 MHz, DMSO-d6): δ -112.21 (s). HRMS (ESI): m/z [M+H]+ calcd for
C13H14ClFN5O 309.7294 found 310.0865.
4.3.9. (1S)-2-[(4-Chloro-6-morpholino-1,3,5-triazin-2-yl)amino]-1-phenyl-ethanol (4i)
Compound 4i was prepared from 2 (0.3 g, 1.276 mmol, 1 equiv) and (s)-2-amino-1-phenyl-
ethanol (i) (0.175g, 1.276 mmol 1 equiv) for 60 min following the general procedure 2.
Compound 4i was obtained after recrystallization in ethanol then pentane as a pale yellow
1
solid (0.379 g, 88 %); Mp: 120 °C; -29.6 (c 1.45 in CH2Cl2); ; H NMR (250 MHz,
CDCl3): δ 7.40 – 7.21 (m, 5H), 6.27 (s, 1H), 4.87 (dd, J = 8.1, 3.5 Hz, 1H), 3.91 – 3.38 (m,
10H); 13C NMR (63 MHz, CDCl3):δ 169.0, 165.9, 164.3, 141.7, 128.6, 128.0, 127.0, 125.9,
73.4, 66.4, 48.5, 44.5; HRMS (ESI): m/z [M+H]+ calcd for C15H19ClN5O2 336.1228 found.
336.1221.
4.3.10. (1R)-2-[(4-Chloro-6-morpholino-1,3,5-triazin-2-yl)amino]-1-phenyl-ethanol (4j)
Compound 4j was prepared from 2 (0.3 g, 1.276 mmol, 1 eq) and (R)-2-amino-1-phenyl-
ethanol j (0.175g, 1.276 mmol, 1 equiv) for 60 min following the general procedure 2.
Compound 3j was obtained after recrystallization in ethanol then pentane as a white solid.
(0.382 g, 89%); Mp: 142°C; +34.9 (c 2.36 in CH2Cl2); 1H NMR (250 MHz, CDCl3): δ
7.40 – 7.21 (m, 5H), 6.16 (s, 1H), 4.88 (dd, J = 8.0, 3.5 Hz, 1H), 3.94 – 3.36 (m, 10H) ; 13C
NMR (63 MHz, CDCl3):δ 165.9, 164.3, 141.7, 128.6, 128.0, 125.9, 73.5, 66.4, 48.5, 44.5.
HRMS (ESI): m/z [M+H]+ calcd for C15H19ClN5O2 336.1228 found. 336.1222.
4.3.11. tert-butyl4-[[4-chloro-6-(4-methoxyanilino)-1,3,5-triazin-2-yl]amino]piperidine-
1-carboxylate (5a)
Compound 5a was prepared from 3 (0.3 g, 0.8614 mmol, 1 equiv) and and p-anisidine (a)
(0.106 g, 0.8614 mmol, 1 equiv) for 30 min following the general procedure 2. The desired
product was obtained after recrystallization in ethanol then pentane as a white solid (0.317 g,
85%); Mp:114 °C; 1H NMR (400 MHz, CDCl3): δ 7.44 (d, J = 8.7 Hz, 1H), 7.39 (d, J = 8.7 Hz,

1H), 6.89 (d, J = 8.9 Hz, 2H), 5.52 (s,1H), 4.15 – 3.93 (m, 3H), 3.83 (s, 3H), 2.93 (t, J = 12.1
13
Hz, 2H), 2.01 (d, J = 12.1 Hz, 2H), 1.48 (s, 9H); C NMR (101 MHz, CDCl3):δ 156. 6, 154.6,
123.3, 122.6, 114.2, 79.8, 55.5, 48.0, 32.1, 31.52, 28.4. HRMS (ESI): m/z [M+H]+ calcd for
C20H28ClN6O3 435.1912 found 435.1906.
4.3.12. tert-Butyl4-[[4-chloro-6-(3-methoxyanilino)-1,3,5-triazin-2-yl]amino]piperidine-
1-carboxylate (5b)
Compound 5b was prepared from 3 (0.3 g, 0.8614 mmol, 1 equiv) and and m-anisidine (b)
(0.106g, 0.8614 mmol, 1 equiv) for 30 min following the general procedure 2. The desired
product was obtained after recrystallization in ethanol then pentane as a brown solid (0.331
g, 88 %); Mp: 108 °C ;1H NMR (250 MHz, CDCl3):δ 7.32 (t, J = 2.2 Hz, 1H), 7.25 – 7.16 (m,
1H), 6.97 (d, J = 8.0 Hz, 1H), 6.67 – 6.60 (m, 1H), 5.42 (s, 1H), 4.12 – 3.92 (m, 3H), 3.78 (s,
3H), 2.89 (q, J = 9.4, 7.3 Hz, 2H), 2.04 – 1.94 (m, 2H), 1.44 (s, 9H) ;13C NMR (63 MHz,
CDCl3):δ 160.1, 154.6, 138.8, 129.6, 112.6, 79.8, 55.4, 32.0, 31.6, 28.4. HRMS (ESI): m/z
[M+H]+ calcd for C20H28ClN6O3 435.1912 found 435.1907.
4.3.13.
tert-Butyl
4-[[4-chloro-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazin-2-
yl]amino]piperidi ne-1-carboxylate (5c)
Compound 5c was prepared from 3 (0.3 g, 0.8614 mmol, 1 equiv) and p-
trifluoromethoxyaniline (c) (0.153 g, 0.8614 mmol, 1 equiv) for 30 min following the general
procedure 2. Compound 5c was obtained solid after recrystallization in ethanol then pentane
1
as a white (0.33 g, 78%); Mp: 110 °C; H NMR (250 MHz, CDCl3) :δ 7.60 – 7.47 (m, 2H),
7.15 (dd, J = 8.6, 3.3 Hz, 2H), 5.49 (d, J = 55.4 Hz, 1H), 3.98 (dd, J = 33.5, 15.3 Hz, 3H),
2.89 (t, J = 12.4 Hz, 2H), 1.98 (dd, J = 12.4, 3.7 Hz, 2H), 1.44 (s, 9H). 13C NMR (63 MHz,
CDCl3):δ 154.6, 145.2, 136.3, 122.0, 121.7, 121.6, 79.9, 79.8, 48.8, 32.08, 31.5, 28.4 ; 19F
NMR (235 MHz, CDCl3):
δ
-58.12 (s). HRMS (ESI): m/z [M+H]+ calcd for
C20H24ClF3N6O3 489.1630 found 489.1623
4.3.14.
tert-Butyl4-[[4-chloro-6-[3-(trifluoromethoxy)anilino]-1,3,5-triazin-2-
yl]amino]piperidine-1-carboxylate (5d)
Compound 5d was prepared from 3 (0.3 g, 0.8614 mmol, 1 equiv) and m-
trifluoromethoxyaniline (d) (0.153g, 0.8614 mmol, 1 equiv) for 1.5 h, following the general
procedure 2. Compound 5d was obtained as a white solid after recrystallization in ethanol
1
then pentane (0,31 g, 74 %); Mp: 107 °C; H NMR (400 MHz, CDCl3): δ 8.01 – 7.82 (m, 1H),
7.68 – 7.53 (m, 1H), 7.42 – 7.18 (m, 1H), 6.99 (d, J = 8.0 Hz, 1H), 5.70 – 5.32 (m, 1H), 4.21 –
13
3.97 (m, 2H), 3.01 – 2.87 (m, 2H), 2.19 (s, 1H), 2.08 – 1.99 (m, 2H), 1.49 (s, 9H). C NMR
(101 MHz, CDCl3): δ 165.1, 164.0, 154.6, 149.5, 139.2, 129.9, 119.2, 118.5, 118.1, 116.0,
113.2, 79.9, 48.6, 48.2, 31.6, 28.; HRMS (ESI): m/z [M+H]+ calcd for
C20H25ClF3N6O3 489.1630 found 489.1623.
4.3.15. tert-Butyl 4-[[4-chloro-6-(4-nitroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (5e)
Compound 5e was prepared from 3 (0.35 g, 1.005 mmol, 1 equiv) and p-nitroaniline (e)
(0.139g, 1.005 mmol, 1 equiv) for 3 h, following the general procedure 2. Compound (5e)
was obtained as a yellow solid after recrystallization in ethanol then pentane (0.21 g, 40 %);
Mp: 173 °C; 1H NMR (400 MHz, DMSO-d6):δ 10.82 (s, 1H), 8.64 (s, 1H), 8.23 (dd, J = 34.0
Hz, 9.1 Hz, 2H), 7.99 (dd, J = 31.5, 9.0 Hz, 2H), 3.94 (d, J = 12.0 Hz, 2H), 2.93 (d, J = 25.9
Hz, 2H), 2.03 – 1.77 (m, 2H), 1.41 (s, 9H); 13C NMR (101 MHz, DMSO-d6): δ 164.0, 154.4,

154.3, 152.2, 151.8, 145.7, 145.6, 142.4, 125.3, 125.1, 120.0, 115.6, 115.5, 79.2, 48.2, 31.6,
31.1, 28.5; HRMS (ESI): m/z [M+H]+ calcd for C19H24ClN7O4 450.1657 found 450.1651.
4.3.16. tert-Butyl 4-[[4-chloro-6-(3-nitroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (5f)
Compound 5f was prepared from 3 (0,35 g, 1,005 mmol, 1 equiv) and m-nitroaniline (f)
(0.139g, 1.005 mmol, 1 equiv) for 1.5 h following the general procedure 2. Compound 5f was
obtained as a yellow solid after recrystallization in ethanol then pentane (0.32 g, 71%);
1
Mp:165 °C; H NMR (400 MHz, CDCl3):δ 9.04 (bs, 1H), 8.62 (s, 1H), 8.00 – 7.66 (m, 2H),
7.64 – 7.36 (m, 2H), 5.76 – 5.40 (m, 1H), 4.13 – 3.79 (m, 1H), 2.14 – 1.90 (m, 2H), 1.79 –
1.59 (m, 3H), 1.50 – 1.14 (m, 5H), 1.51 (s, 9H); 13C NMR (101 MHz, CDCl3): δ 169.1, 165.0,
164.2, 148.8, 139.4, 129.6, 125.4, 118.4, 115.1, 50.5, 33,0, 32.8, 25.6, 24.9, 24.6; HRMS
(ESI): m/z [M+H]+ calcd for C19H25ClN7O4 450.1657 found 450.1651.
4.3.17. tert-Butyl 4-[[4-chloro-6-(4-fluoroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (5g)
Compound 5g was prepared from 3 (0.4 g, 1.159 mmol, 1 equiv) and p-fluoroaniline (g)
(0.163g, 1.159 mmol, 1 equiv) for 30 min following the general procedure 2. Compound 5g
was obtained as a brown solid after recrystallization in ethanol then pentane (0.437 g, 89 %);
Mp: 181 °C; 1H NMR (400 MHz, CDCl3): δ 7.54 – 7.44 (m, 2H), 7.09 – 7.01 (m, 2H), 5.60 (s,
1H), 4.01 (d, J = 50.6 Hz, 3H), 2.93 (t, J = 12.0 Hz, 2H), 2.11 – 1.95 (m, 2H), 1.48 (s, 9H); 13C
NMR (101 MHz, CDCl3):δ 165.3, 164.2, 160.7, 158.2, 154.7, 154.6, 133.5, 133.4, 123.1,
123.0, 122.7, 122.6, 115.7, 115.7, 115.5, 115.4, 79.9, 79.8, 48.1, 34.1, 32.0, 31.5, 28.4; 19F
NMR (376 MHz, CDCl3): δ -117.84; HRMS (ESI): m/z [M+H]+ calcd for C18H24N6 423.1712
found 423.1706.
4.3.18. tert-Butyl 4-[[4-chloro-6-(3-fluoroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (5h)
Compound 5h was prepared from 2 (0.4 g, 1.159 mmol, 1 eq) and m-fluoroaniline h (0.163 g,
1.159 mmol, 1eq) for 30 min following the general procedure 2. Compound 5h was obtained
1
after recrystallization in ethanol then pentane as a white solid (0.441 g, 90%); Mp: 172°C; H
NMR (400 MHz, CDCl3) δ 7.09 (t, J = 7.1 Hz, 1H), 6.82 (qd, J = 7.9, 2.1 Hz, 1H), 4.20-4.05
(m, 3H), 3.00 – 2.92 (m, 2H), 2.05 (t, J = 13.5 Hz, 2H), 1.76 (s, 1H), 1.49 (s, 9H). 13C NMR
(101 MHz, CDCl3) δ 165.3, 165.1, 164.1, 164.0, 163.6, 161.7, 154.7, 154.6, 139.4, 139.3,
130.0, 129.9, 115.7, 115.4, 110.7, 110.5, 108.1, 107.9, 107.8, 107.7, 79.9, 79.8, 48.1, 48.2,
34.1, 32.0, 31.5, 28.4; 19F NMR (376 MHz, CDCl3):δ -111.27 (s); HRMS (ESI): m/z [M+H]+
calcd for C18H24N6 423.1712 found 423.1706.
4.4. General procedure 3
In round bottom flask, 1 equiv of disubtituted triazine, 1.1 equiv of KCN, and 1.02 equiv of
DABCO were added in DMSO/H2O (91/9). The reaction mixture was stirred for 48h at room
temperature. After completion of the reaction, DMSO was evaporated and the resulting solid
was dissolved in EtOAc or CH2Cl2 and washed with water (2 x 40 mL) then with brine
solution (2 x 30 mL). The organic layer was dried with MgSO4 and purified by silica gel
column chromatography to give the desired product.
4.4.1. 4-(4-Methoxyanilino)-6-(morpholin-4-yl)-1,3,5-triazine-2-carbonitrile (7a)

Compound 7a was prepared from 4a (0.1 g, 0.311 mmol, 1 equiv) following the general
procedure 3. the desired product was obtained after purification on column chromatography
1
(PE/EtOAc: 8/2 to 6/4) as a yellow solid (60 mg, 62 %); Mp: 184°C; H NMR (400 MHz,
CDCl3): δ 7.40 (d, J = 9.0 Hz, 2H), 6.92 (d, J = 9.0 Hz, 2H), 3.87 – 3.83 (m, 2H), 3.81 (s, 3H),
3.78 – 3.76 (m, 2H), 3.75 – 3.69 (m, 4H); 13C NMR (101 MHz, CDCl3): δ 163.7, 156.7, 130.1,
122.8, 115.1, 114.2, 66.6, 66.4, 55.5, 44.1, 43.7; HRMS (ESI): m/z [M+H]+ calcd for
C15H17N6O2 313.1314 found 313.1409
4.4.2. 4-(3-Methoxyanilino)-6-(morpholin-4-yl)-1,3,5-triazine-2-carbonitrile (7b)
Compound 7b was prepared from 4b (0.1 g, 0.311 mmol, 1 equiv), following the general
procedure 3 for 48h. The desired product was obtained after purification on column
1
chromatography (PE/EtOAc: 8/2 to 6/4) as a yellow solid (0.082g, 82%); Mp: 179 °C; H
NMR (400 MHz, CDCl3):δ 7.27 – 7.21 (m, 2H), 7.01 (dd, J = 8.0, 2.0 Hz, 1H), 6.69 (dd, J =
8.0, 2.0 Hz, 1H), 3.88 – 3.85 (m, 4H), 3.81 (s, 3H), 3.77 – 3.73 (m, 4H); 13C NMR (101 MHz,
CDCl3):δ 163.6, 160.1, 152.2, 138.4, 129.8, 115.0, 112.8, 109.7, 106.7, 66.6, 66.4, 55.3,
44.2, 43.8. HRMS (ESI): m/z [M+H]+ calcd for C15H17N6O2 313.1314 found 313.1409.
4.4.3.
4-(Morpholin-4-yl)-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazine-2-carbonitrile
(7c)
Compound 7c was prepared from 4c (0.1 g, 0.266 mmol, 1 equiv) following the general
procedure 3 for 48h. the desired Compound was obtained after purification on column
1
chromatography (PE/EtOAc: 8/2 to 6/4) as a yellow solid (62 mg, 60 %); Mp: 298 °C; H
NMR (400 MHz, CDCl3):δ 7.54 (d, J = 8.9 Hz, 2H), 7.22 (d, J = 8.9 Hz, 2H), 3.88 – 3.85 (m,
13
2H), 3.84 – 3.79 (m, 2H), 3.77 – 3.73 (m, 4H); C NMR (101 MHz, CDCl3):δ 163.7, 152.4,
145.5, 136.1, 121.9, 121.9, 115.1, 66.7, 66.5, 44.3, 44.0, 29.8; 19F NMR (376 MHz, CDCl3) :δ
-58.10 (s) ; HRMS (ESI): m/z [M+H]+ calcd for C15H14F3N6O2 367.1131 found 367.1124
4.4.4.
4-(Morpholin-4-yl)-6-[3-(trifluoromethoxy)anilino]-1,3,5-triazine-2-carbonitrile
(7d)
Compound 7d was prepared from 4d (0.1 g, 0.266 mmol, 1 equiv) following the general
procedure 3. Compound 7d was obtained after purification on column chromatography
1
(PE/EtOAc: 8/2 to 6/4) as a white solid (0.096 g, 98 %); Mp: 164 °C; H NMR (400 MHz,
CDCl3):δ 7.77 (s, 1H), 7.35 (t, J = 8.1 Hz, 1H), 7.27 (s, 1H), 6.98 (d, J = 8.1 Hz, 1H), 3.88 (t,
13
J = 4.6 Hz, 2H), 3.83 (t, J = 4.6 Hz, 2H), 3.79 – 3.75 (m, 4H); C NMR (101 MHz, CDCl3):δ
163.7, 152.4, 149.6, 139.1, 130.2, 121.8, 118.6, 116.4, 115.1, 113.2, 66.7, 66.5, 44.4, 44.0,
41.0; 19F NMR (376 MHz, CDCl3):δ -57.68 (s); HRMS (ESI): m/z [M+H]+ calcd for
C15H14F3N6O2 367.1131 found 367.1124.
4.4.5. 4-(Morpholin-4-yl)-6-(4-nitroanilino)-1,3,5-triazine-2-carbonitrile (7e)
Compound 7e was prepared from 4e (0.1 g, 0,3 mmol, 1 equiv) following the general
procedure 3. the desired product 7e was obtained after purification on column
1
chromatography (PE/EtOAc : 8/2 to 6/4) as a yellow solid (78 mg, 79%); Mp: 294°C; H NMR
(400 MHz, DMSO-d6):δ 10.83 (s,1H), 8.24 (dd, J = 9.9, 2.6 Hz, 2H), 7.90 (dd, J = 9.9, 2.6 Hz,
2H), 3.82 – 3.74 (m, 4H), 3.72 – 3.66 (m, 4H); 13C NMR (101 MHz, DMSO-d6): δ 163.0,
151.6, 145.0, 142.1, 125.0, 119.9, 115.2, 99.6, 65.8, 30.6; HRMS (ESI): m/z [M+H]+ calcd for
C14H14N7O3 328.1159 found 328.1152.
4.4.6. 4-(Morpholin-4-yl)-6-(3-nitroanilino)-1,3,5-triazine-2-carbonitrile (7f)

Compound 7f was prepared from 4f (0.26 g, 0.776 mmol, 1 equiv) following the general
procedure 3. The desired product was obtained after purification on column chromatography
1
(PE/EtOAc: 8/2 to 6/4) as a yellow solid (0.187g, 73%); Mp: 286 °C; H NMR (400 MHz,
DMSO-d6): δ 10.74 (s, 1H) 8.83 (s, 1H), 7.91 (t, J = 7.0 Hz, 2H), 7.64 (t, J = 7.0 Hz, 1H),
3.74– 3.55 (m, 8H); 13C NMR (101 MHz, DMSO-d6): δ 163.0, 151.6, 145.0, 142.1, 125.0,
119.9, 115.2, 99.6, 65.8, 44.0, 43.9, 30.8; HRMS (ESI): m/z [M+H]+ calcd for
C14H14N7O3 328.1159 found 328.1152.
4.4.7. 4-(4-Fluoroanilino)-6-morpholino-1,3,5-triazine-2-carbonitrile (7g)
Compound 7g was prepared from 4g (0.3 g, 0.969 mmol, 1 equiv) and following the general
procedure 3. The desired product was obtained after recrystallization in ethanol then pentane
1
as a white solid (0.225 g, 77%); Mp : 272 °C; H NMR (250 MHz, CD3OD): δ 7.58 (dd, J =
9.2, 4.9 Hz, 2H), 7.11 – 6.91 (m, 2H), 3.84 – 3.78 (m, 4H), 3.74 – 3.68 (m, 4H); 13C NMR
(101 MHz, CDCl3):δ 164.6, 163.8, 145.1, 136.4, 121.7, 121.5, 121.4, 66.6, 66.5, 44.1; 19F
NMR (376 MHz, CDCl3): δ -117.46. HRMS (ESI): m/z [M+H]+ calcd for C14H14FN6O 301.1214
found 301.1208
4.4.8. 4-(3-Fluoroanilino)-6-morpholino-1,3,5-triazine-2-carbonitrile (7h)
Compound 7h was prepared from 4h (0.3 g, 0.969 mmol, 1 equiv) following the general
procedure 3. The desired product was obtained after recrystallization in ethanol then pentane
(0.238 g, 82%) as a yellow solid; Mp: 265 °C; 1H NMR (400 MHz, CDCl3): δ 7.50 (dt, J = 8.3,
2.3 Hz, 1H), 7.32 – 7.27 (m, 1H), 7.13 (d, J = 8.3 Hz, 1H), 6.83 (td, J = 8.3, 2.3 Hz, 1H), 3.88
(t, J = 4.9 Hz, 2H), 3.84 (t, J = 4.9 Hz, 2H), 3.77 (t, J = 6.1 Hz, 4H); 13C NMR (101 MHz,
CDCl3): δ 164.1, 163.6, 161.7, 152.2, 138.9, 138.8, 130.2, 130.1, 115.7, 115.7, 115.0, 111.1,
110.8, 108.0, 107.7, 66.6, 66.4, 44.2, 43.9; 19F NMR (376 MHz, CDCl3): δ -112.23 (s); HRMS
(ESI): m/z [M+H]+ calcd for C14H14FN6O 301.1214 found 301.1208.
4.4.9.
4-[[(2S)-2-Hydroxy-2-phenyl-ethyl]amino]-6-morpholino-1,3,5-triazine-2-
carbonitrile (7i)
Compound 7i was prepared from 4i (0.25 g, 0.746 mmol, 1 equiv) following the general
procedure 3. The desired product was obtained as a white solid after recrystallization in
1
ethanol then pentane (0.184 g, 76 %); Mp: 163 °C; +30.1 (c 1.14 in CH2Cl2); H NMR
(400 MHz, CDCl3): δ 7.36 (d, J = 7.27 Hz, 5H), 5.01 – 4.85 (m, 1H), 3.89 – 3.65 (m, 9H),
13
3.52– 3.18 (m, 1H); C NMR (101 MHz, CDCl3): δ 165.0, 163.4, 151.8, 141.5, 128.7, 128.1,
125.9, 115.2, 73.3, 73.05, 66.6, 66.4, 48.2, 44.0, 43.5, 40.7; HRMS (ESI): m/z [M+H]+ calcd
for C16H19N6O2 327.1570 found 327.1567.
4.4.10.
4-[[(2R)-2-Hydroxy-2-phenyl-ethyl]amino]-6-morpholino-1,3,5-triazine-2-
carbonitrile (7j)
Compound 7j was prepared from 4j (0.25 g, 0.746 mmol, 1 equiv) and KCN (1.1 equiv)
following the general procedure 3. Compound 7j was obtained after recrystallization in
ethanol then pentane as a yellow solid (0.18 g,74 %); Mp : 176 °C ; -34.9 (c 2.36 in
CH2Cl2); 1H NMR (400 MHz, CDCl3):δ 7.40 (d, J = 6.6 Hz, 4H), 7.35 – 7.26 (m, 1H), 4.93
(dd, J = 8.2, 3.6 Hz, 1H), 3.96 – 3.65 (m, 9H), 3.52– 3.18 (m, 1H). 13C NMR (101 MHz,
CDCl3) δ 165.5, 165.0, 163.4, 151.8, 141.5, 128.7, 128.1, 127.0, 125.9, 115.2, 73.7, 73.0,
66.6, 66.4, 48.3, 48.2, 44.0, 43.5; HRMS (ESI): m/z [M+H]+ calcd for C16H19N6O2 327.1570
found 327.1567.

4.4.11. tert-Butyl 4-[[4-cyano-6-(4-methoxyanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (8a)
Compound 8a was prepared from 5a (0.25 g, 0.575 mmol, 1 equiv) following the general
procedure 3. The desired product was obtained after recrystallization in ethanol then pentane
1
as a yellow solid (0.204 g, 85%); Mp : 168 °C; H NMR (400 MHz, CDCl3):δ 7.42 (dd, J = 8.5
Hz, 2H), 6.98 – 6.85 (m, 2H), 5.58 (s, 1H), 4.15 – 3.90 (m, 3H), 3.83 (d, J = 4.7 Hz, 3H), 2.93
(t, J = 13.0Hz, 2H), 2.01 (ddd, J = 13.0, 8.7, 4.7 Hz, 2H), 1.49 (s, 9H); 13C NMR (101 MHz,
CDCl3):δ 157.0, 154.7, 123.4, 122.6, 114.2, 79.9, 79.8, 55.5, 40.9, 32.0, 31.4, 28.4; HRMS
(ESI): m/z [M+H]+ calcd for C21H28N7O3 426.2254 found 426.2247.
4.4.12. tert-Butyl 4-[[4-cyano-6-(3-methoxyanilino)-1,3,5-triazin-2-yl]amino]piperidine-
1-carboxylate (8b)
Compound 8b was prepared from 5b (0.25 g, 0.575 mmol, 1 equiv) following the general
procedure 3. The desired compound 8b was obtained after recrystallization in ethanol then
1
pentane as a yellow solid (0.171 g, 70%); Mp : 149 °C; H NMR (400 MHz, CDCl3): δ 7.30 –
7.24 (m, 2H), 7.04 (d, J = 8.0 Hz, 1H), 6.71 (dd, J = 8.2, 2.1 Hz, 1H), 5.56 (s, 1H), 4.20 –
3.96 (m, 3H), 3.84 (s, 3H), 3.01 – 2.86 (m, 2H), 2.03 (t, J = 14.7 Hz, 2H), 1.49 (s, 9H); 13C
NMR (101 MHz, CDCl3):δ 164.6, 160.2, 160.1, 154.7, 154.6, 138.4, 138.2, 129.8, 129.7,
113.2, 112.8, 79.9, 79.8, 55.4, 55.4, 48.5, 40.9, 31.9, 31.5, 28.4; HRMS (ESI): m/z [M+H]+
calcd for C21H28N7O3 426.2254 found 426.2247.
4.4.13.
tert-Butyl
4-[[4-cyano-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazin-2-
yl]amino]piperidine-1-carboxylate (8c)
Compound 8c was prepared from 5c (0.25 g, 0.510 mmol, 1 equiv) following the general
procedure 3. 8c was obtained after recrystallization in ethanol then pentane as a yellow solid
1
(0.218 g, 89%); Mp : 170°C; H NMR (250 MHz, CDCl3): δ 7.60 – 7.47 (m, 2H), 7.15 (dd, J =
8.6, 3.3 Hz, 2H), 5.50-5.49 (m, 1H), 4.10-3.98 (m, 3H), 2.89 (t, J = 12.4 Hz, 2H), 1.98 (dd, J =
12.4, 3.7 Hz, 2H), 1.44 (s, 9H); 13C NMR (63 MHz, CDCl3):δ 164.2, 154.6, 145.2, 136.3,
122.0, 121.7, 121.6, 79.9, 79.8, 48.2, 32.1, 31.5, 28.4; 19F NMR (235 MHz, CDCl3):δ -58.12;
HRMS (ESI): m/z [M+H]+ calcd for C21H25F3N7O3 480.1972 found 480.1968.
4.4.14.
tert-Butyl
4-[[4-cyano-6-[3-(trifluoromethoxy)anilino]-1,3,5-triazin-2-
yl]amino]piperidine-1-carboxylate (8d)
Compound 8d was prepared from 5d (0.25 g, 0.510 mmol, 1 equiv) following the general
procedure 3. The desired product 8d was obtained after recrystallization in ethanol then
1
pentane as a yellow solid (0.236 g, 96 %); Mp : 158 °C; H NMR (400 MHz, CDCl3): δ 8.01 –
7.82 (m, 1H), 7.68 – 7.53 (m, 1H), 7.42 – 7.18 (m, 2H), 6.99 (d, J = 8.0 Hz, 1H), 5.70 – 5.32
(m, 1H), 4.21 – 3.97 (m, 3H), 3.01 – 2.87 (m, 2H), 2.05-2.02 (m, 2H), 2.08 – 1.99 (m, 2H),
13
1.45 (s, 9H); C NMR (101 MHz, CDCl3):δ 165.1, 164.0, 154.6, 149.5, 139.2, 129.9, 119.2,
118.5, 118.1, 116.0, 113.2, 79.9, 48.6, 48.2, 31.6. HRMS (ESI): m/z [M+H]+ calcd for
C21H25F3N7O3 480.1972 found 480.1968
4.4.15. tert-Butyl 4-[[4-cyano-6-(3-nitroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (8f)

Compound (8f) was prepared from 5f (0.25 g, 0.555 mmol, 1 equiv) following the general
procedure 3. The desired product was obtained after purification on column chromatography
1
(PE/EtOAc : 8/2) as a yellow solid (0.11 g, 45%); Mp : 173°C; H NMR (400 MHz, CDCl3): δ
8.09 – 7.93 (m, 1H), 7.90 – 7.35 (m, 3H), 5.95 – 5.42 (m, 1H), 3.99 (bs, 1H), 2.24 – 1.92 (m,
2H), 1.82 – 1.64 (m, 3H), 1.55-1.40 (m, 4H), 1.48 (s, 9H); 13C NMR: (101 MHz, CDCl3):δ
164.4, 164.2, 152.3, 148.8, 138,9 129.9, 129.8, 118.8, 115.1, 114.8, 50.4, 33.0, 40.9, 32.0,
31.4, 28.4; HRMS (ESI): m/z [M+H]+ calcd for C20H25N8O4 441.2000 found 441.1993
4.4.16. tert-Butyl 4-[[4-cyano-6-(4-fluoroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (8g)
Compound 8g was prepared from 5g (0.3 g, 0.726 mmol, 1 equiv) following the general
procedure 3. The desired product after purification on column chromatography (PE/EtOAc :
8/2) was obtained as a white solid (0.265 g, 88%); Mp: 178 °C; 1H NMR (400 MHz, CDCl3): δ
7.42 (dd, J = 8.5 Hz, 2H), 6.91 (dd, J = 8.5 Hz, 2H), 5.58 (s, 1H), 4.15 – 3.90 (m, 3H), 3.83
(d, J = 4.7 Hz, 3H), 2.93 (t, J = 12.6 Hz, 2H), 2.01 (ddd, J = 12.6, 8.7, 4.7 Hz, 2H), 1.47 (s,
9H); 13C NMR (101 MHz, CDCl3): δ 157.0, 154.7, 123.4, 122.6, 114.2, 79.9, 79.8, 55.5, 40.9,
32.0, 31.4, 28.4; HRMS (ESI): m/z [M+H]+ calcd C20H25FN7O2 414.2055 found 414.2048.
4.4.17. tert-Butyl 4-[[4-cyano-6-(3-fluoroanilino)-1,3,5-triazin-2-yl]amino]piperidine-1-
carboxylate (8h)
Compound 8h was prepared from 5h (0.3 g, 0.726 mmol, 1 equiv) following the general
procedure 3. The desired products was obtained as after purification on column
1
chromatography (PE/EtOAc : 8/2) a white solid (0.22 g,73 %); Mp: 163 °C; H NMR (400
MHz, DMSO-d6): δ 10.43 (s, 1H), 7.52 (dt, J = 11.8, 2.5 Hz, 1H), 7.45 (d, J = 8.5 Hz, 1H),
7.42 – 7.31 (m, 1H), 6.88 (td, J = 8.5, 2.5 Hz, 1H), 3.76 – 3.72 (m, 4H), 3.47– 3.40 (m, 4H),
1.43 (s, 9H); 13C NMR (101 MHz, DMSO-d6):δ 163.2, 163.0, 162.7, 162.6, 160.8, 153.7,
151.3, 140.2, 140.1, 130.4, 130.3, 116.1, 115.2, 109.8, 109.6, 107.2, 106.9, 79.3, 43.1, 43.0,
28.0; HRMS (ESI): m/z [M+H]+ calcd C20H25FN7O2 414.2055 found 414.2050.
4.5. General procedure 4
To the solution of Boc-piperidine derivative in CH2Cl2 was added TFA (0.6mL) and reaction
mixture was stirred till completion, under N2 at room temperature. Then, the mixture was
concentrated and the residue was dissolved in CH2Cl2 and satured NaHCO3 aqueous
solution was added. The aqueous phase was extracted with CH2Cl2. Combined organic
phases were washed with water, dried over MgSO4 and concentrated. The crude was
purified by column chromatography (CH2Cl2/ MeOH) to give the desired product.
4.5.1. 4-(4-Methoxyanilino)-6-(4-piperidylamino)-1,3,5-triazine-2-carbonitrile (9a)
Compound 9a was prepared from 8a (0.16 g, 0.376 mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
1
(DCM/MeOH: 8/2) as a white solid (0.1098 g, 90 %); Mp: 171 °C; H NMR (250 MHz,
CD3OD): δ 7.54 (s, 2H), 6.93 – 6.82 (m, 2H), 3.78 (d, J = 2.7 Hz, 3H), 3.72 (t, J = 6.7 Hz,
1H), 3.18 – 3.00 (m, 2H), 2.83 – 2.57 (m, 2H), 2.10 – 1.81 (m, 3H), 1.48 (d, J = 11.7 Hz, 4H);
13C NMR (101 MHz, CDCl3):δ 157.1, 154.8, 129.9, 123.5, 122.8, 114.8, 80.1, 55.6, 41.1,
32.1, 31.5; HRMS (ESI): m/z [M+H]+ calcd for C16H20N7O 326.1730 found 326.1723.
4.5.2. 4-(3-Methoxyanilino)-6-(4-piperidylamino)-1,3,5-triazine-2-carbonitrile (9b)

Compound 9b was prepared from 8b (0.16 g, 0.376 mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
1
(DCM/MeOH: 8/2) as a white solid (0.095 g, 78 %); Mp : 177 °C; H NMR (250 MHz,
CD3OD): δ 7.39 (s, 1H), 7.25 – 7.11 (m, 2H), 6.66 – 6.57 (m, 1H), 4.05 – 3.89 (m, 1H), 3.77
(d, J = 2.2 Hz, 3H), 3.18 – 2.73 (m, 2H), 3.04 – 2.49 (m, 2H), 2.08 – 1.91 (m, 2H), 1.55 – 1.44
(m, 3H), 1.28 – 1.24 (m, 1H); 13C NMR (101 MHz, CDCl3):δ 164.7, 160.3, 160.2, 154.8,
138.5, 138.4, 129.9, 113.3, 112.9, 80.1, 55.5, 48.6, 32.1, 31.6; HRMS (ESI): m/z [M+H]+
calcd for C16H20N7O 326.1730 found 326.1723.
4.5.3.
4-[(Piperidin-4-yl)amino]-6-[4-(trifluoromethoxy)anilino]-1,3,5-triazine-2-
carbonitrile (9c)
Compound 9c was prepared from 8c (0.16 g, 0.344mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
(DCM/MeOH: 9/1) as a white solid (0.125 g, 96%); Mp: 185 °C; 1H NMR (250 MHz,
CD3OD):δ 7.89-7.76 (m, 2H), 7.22-7.09 (m, 2H), 3.13-3.04 (m, 2H), 2.74-2.61 (m, 2H), 2.12-
13
1.83 (m, 2H), 1.55-1.39 (m, 3H); C NMR (101 MHz, CDCl3): δ 165.0, 164.2, 152.3, 140.8,
126.4, 126.4, 126.3, 125.5, 122.8, 120.2, 119.9, 115.1, 50.2, 41.0, 33.1; 19F NMR (235 MHz,
CD3OD):δ -73,46 (s); HRMS (ESI): m/z [M+H]+ calcd for C16H17F3N7O 380.1441 found
380.1441.
4.5.4. 4-(4-Piperidylamino)-6-[3-(trifluoromethoxy)anilino]-1,3,5-triazine-2-carbonitrile
(9d)
Compound 9d was prepared from 8d (0.16 g, 0.344mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
1
(DCM/MeOH: 9/1) as a white solid (0.073 g, 56 %); Mp: 210 °C; H NMR (250 MHz,
CD3OD):δ 7.92 – 7.62 (m, 1H), 7.53 – 7.31 (m, 2H), 6.98 (d, J = 7.4 Hz, 1H), 3.23 – 3.02 (m,
2H), 2.77 – 2.63 (m, 2H), 2.03–1.99 (m, 2H), 1.66 – 1.35 (m, 4H);13C NMR (101 MHz,
CDCl3): δ 164.4, 164.2, 152.8, 152.3, 148.8, 138.9, 138.8, 129.9, 125.6, 126.4, 125.1, 118.9,
115.4, 115.1, 114.8, 50.3, 41.02, 32.7; 19F NMR (235 MHz, CD3OD):δ -73,5 (s); HRMS (ESI):
m/z [M+H]+ calcd for C16H17F3N7O 380.1441 found 380.1440.
4.5.5. 4-(3-Nitroanilino)-6-(4-piperidylamino)-1,3,5-triazine-2-carbonitrile (9f)
Compound 9f was prepared from 8f (0.16 g, 0.363 mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
1
(DCM/MeOH: 8/2) as a yellow solid (0.09 g, 72 %); Mp: 205 °C; H NMR (250 MHz, DMSO-
d6):δ 8.77 (d, J = 25 Hz, 1H), 7.92 – 7.76 (m, 2H),7.62– 7.58 (t, J = 8.2 Hz, 1H), 4.11 – 3.77
(m, 4H), 2.83 (s, 2H), 2.04 (s, 2H), 1.95 – 1.66 (m, 2H); 13C NMR (101 MHz, DMSO-d6):δ
163.3, 151.6, 151.3, 143.3, 143.2, 133.0, 133.0, 120.0, 119.8, 115.1, 49.5, 32.1, 31.7; HRMS
(ESI): m/z [M+H]+ calcd for C15H16N8O2 341.1475 found 341.1469
4.5.6. 4-(4-Fluoroanilino)-6-(4-piperidylamino)-1,3,5-triazine-2-carbonitrile (9g)
Compound 9g was prepared from 8g (0.18 g, 0.435 mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
1
(DCM/MeOH: 8/2) as a white solid (0.183g, 74%); Mp: 194 °C; H NMR (250 MHz, DMSO-
d6):δ 7.16 (td, J = 8.8, 5.9 Hz, 4H), 3.76 (s, 1H), 2.94– 2.89 (m, 3H), 1.82– 1.79 (m, 3H),
1.37–1.35 (m,4H), 1.24 (s, 1H); 13C NMR (101 MHz, DMSO-d6):δ 163.4, 163.3, 151.6, 151.3,

143.3, 143.2, 133.0, 133.0, 120.0, 119.8, 119.0, 115.1, 104.5, 49.5, 39.2, 32.1, 31.7; HRMS (ESI):
m/z [M+H]+ calcd for C15H17FN7 314.1526 found 314.1524.
4.5.7. 4-(3-Fluoroanilino)-6-(4-piperidylamino)-1,3,5-triazine-2-carbonitrile (9h)
Compound 9h was prepared from 8h (0.18 g, 0.435 mmol, 1 equiv) following the general
procedure 4. The desired product was obtained after purification on column chromatography
(DCM/MeOH: 8/2) as a white solid (0.17 g, 80%); Mp: 180 °C; 1H NMR (250 MHz, CD3OD):δ
7.89-7.83 (m, 1H), 7.40 – 7.19 (m, 2H), 6.88 – 6.70 (m, 1H), 3.14 – 3.08 (m, 1H), 2.77– 2.67
13
(m, 2H), 2.06 – 1.94 (m, 2H), 1.64 – 1.31 (m, 4H); C NMR (101 MHz, DMSO-d6):δ 163.4,
163.3, 151.6, 151.3, 143.3, 143.2, 133.0, 133.0, 119.8, 119.0, 115.1, 104.5, 104.4, 49.5,
32.1, 31.7; HRMS (ESI): m/z [M+H]+ calcd for C15H17FN7 314.1526 found 314.1524
4.6 Kinetic assays
Human cathepsins B, L and S were purchased from Calbiochem (VWR International,
Pessac, France) while human cathepsin K was expressed in Pichia pastoris as previously
reported.31 Active site concentrations of cysteine cathepsins were determined using L-3-
carboxy-trans-2,3-epoxy-propionyl-leucylamide-(4-guanido)-butane (E-64) (Sigma-Aldrich,
Saint-Quentin Fallavier, France). Enzymatic assays for cathepsins B, L and K were carried
out at 37 °C in their activity buffer (0.1 M sodium acetate buffer, pH 5.5, containing 2 mM
DTT and 0.01% Brij35), using Z-Phe-Arg-AMC (benzyloxycarbonyl-phenylalanyl-arginine-4-
methylcoumarin, Bachem, Bubendorf, Switzerland) as substrate (spectromicrofluorimeter
SpectraMax Gemini, Molecular Devices, Saint Grégoire, France; λex = 350 nm, λem = 460
nm). The same protocol was operated for cathepsin S, except that Z-LR-AMC (Bachem) was
used as substrate. Cathepsins B, K, L and S (1 nM) were incubated in the activity buffer in
the presence of increasing concentrations of inhibitor for 30 min, before measurement of the
residual enzymatic activity. Slopes were calculated and average values of IC50 determined
(software Softmaxpro, Molecular Devices). Assays were performed in triplicate. The inhibition
constant (Ki) of the most potent inhibitor (compound 7c) was determined using the Cheng-
Prusoff equation (Cheng,Y. and Prusoff,W.H. (1973) Biochem. Pharmacol., 22, 3099–3108).
Ki values are expressed as mean ± SEM (n=3).
4.7 Maturation of procathepsin S
Recombinant pro-Cat S was produced according to Sage et al..32 Pro-Cat S (1µg) was
incubated in 0.1 M sodium acetate buffer, pH 4.0, containing 2 mM DTT, 2 mM EDTA
(ethylenediaminetetraacetic acid;) and 0.01% Brij 35 at 26°C for 5 h in the absence or
presence of the following inhibitors (E-64: 100 µM; LHVS (morpholinourea-leucyl-
homophenylalanine-vinyl-sulfone phenyl (Mu-Leu-Hph-VSPh): 100 µM; 7c: 0.01 – 100 µM).
Following incubation, the samples were re-suspended in SDS-PAGE loading buffer, boiled
and separated by 15% SDS-PAGE under reducing conditions. Electrophoretic gels were
colored by Coomassie blue staining.
Acknowledgments: LHVS, an irreversible inhibitor of CatS, was a kind gift from Pr James
H. McKerrow (Skaggs School of Pharmacy and Pharmaceutical Sciences, University of
California, San Diego, CA, USA. Cathepsin K was kindly provided by Pr Dieter Brömme
(University of British Columbia, Vancouver, British Columbia, Canada).

Funding: We acknowledge the Institut National de la Santé et de la Recherche Médicale
(INSERM) for institutional funding. MW holds a doctoral fellowship from la Région Centre-Val
de Loire, France. DW acknowledges financial support from the National Science Centre
(Poland) grant no.[2016/21/N/NZ2/01725]. We thank the LABEX SynOrg [ANR-11-LABX-
0029] and the FéRI (Fédération de recherche en infectiologie de la région Centre-Val de
Loire) for partial financial support.
Competing interests: The authors declare that they have no competing interests.
References
1. Yamashima T. Reconsider Alzheimer's disease by the 'calpain-cathepsin hypothesis'a
perspective review. Prog Neurobio. 2013;105:1-23.
2. Nomura T, Katunuma N. Involvement of cathepsins in the invasion, metastasis and
proliferation of cancer cells. J Med Invest. 2005;52:1-9.
3. Lipton P. Ischemic cell death in brain neurons. Physiol Rev. 1999;79:1431-1568.
4. Chandran K. Endosomal proteolysis of the Ebola virus glycoprotein is necessary for
infection. Science. 2005;308:1643-1645.
5. Rossi A, Deveraux Q, Turk B, Sali A. Comprehensive search for cysteine cathepsins in
the human genome. Biol Chem. 2004;385:363-372.
6. Turk V, Stoka V, Vasiljeva O, Renko M, Sun T, Turk B, Turk D. Cysteine cathepsins:
from structure, function and regulation to new frontiers. Biochim Biophys Acta
2012;1824:68-88.
7. MEROPS: the peptidase database: http://merops.sanger.ac.uk
8. Fengler A, Brandt W. Protein Eng. 1998; 11: 1007-1013.
9. Helali AM, Iti FM, Mohamed IN. Cathepsins in heart disease–chewing on the
heartache? Curr Drug Targets. 2013; 14:1591-1600.
10. Wilkinson RD, Williams R, Scott CJ, Burden RE. Cathepsin S–Dependent Protease–
Activated Receptor-2 Activation: A New Mechanism of Endothelial Dysfunction. Biol
Chem. 2015;396:867-882.
11. Zhang L, Wang H, Xu J. Cathepsin S as a cancer target. Neoplasma. 2015;62:16-26.
12. Perišic´ Nanut M, Sabotic J, Jewett A, Kos J. Cysteine Cathepsins as Regulators of the
Cytotoxicity of NK and T Cells. Front Immunol. 2014;5:616.
13. Lalmanach G, Saidi A, Marchand-Adam S, Lecaille F, Kasabova M. Cysteine
cathepsins and cystatins: from ancillary tasks to prominent status in lung diseases. Biol.
Chem. 2015; 396:111-130.
14. Frizler M, Mertens MD, Guetschow M. Fluorescent nitrile-based inhibitors of cysteine
cathepsins. Bioorg Med Chem Lett. 2012; 22:7715-7718.
15. Fleming FF, Yao LH, Ravikumar PC, Funk L, Shook BC. Nitrile-Containing
Pharmaceuticals: Efficacious Roles of the Nitrile Pharmacophore. J Med Chem. 2010;
53:7902-7917.
16. Plebanek E, Chevrier F, Roy V, Garenne T, Lecaille F, Warszycki D, Bojarski AJ,
Lalmanach G, Agrofoglio LA. Straightforward synthesis of 2,4,6-trisubstituted 1,3,5-
triazine compounds targeting cysteine cathepsins K and S. Eur J Med Chem.

2016;121:12-20.
17. Frizler M, Lohr F, Lülsdorff M, Gütschow M. Facing the gem‐Dialkyl Effect in Enzyme
Inhibitor Design: Preparation of Homocycloleucine‐Based Azadipeptide Nitriles. Chem.
Eur. J. 2011; 17: 11419-11423.
18. Cheng Y, Prusoff W.H. Relationship between the inhibition constant (K1) and the
concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic
reaction.Biochem. Pharmacol. 1973; 22 : 3099–3108.
19. Galibert M, Wartenberg M, Lecaille F, et al. Substrate-derived triazolo- and azapeptides
as inhibitors of cathepsins K and S. Eur. J. Med. Chem. 2018; 144: 201-210.
20. Sastry GM, Adzhigirey M, Day T, Annabhimoju R, Sherman W. Protein and ligand
preparation: parameters, protocols, and influence on virtual screening enrichments. J.
Comput Aid Mol Des. 2013; 27:221-234.
21. Schrodinger Release 2017-3: LigPrep, Schrödinger, LLC, New York, NY 2017.
22. Schrodinger Release 2017-3: Glide, Schrödinger, LLC, New York, NY 2017.
23. K. Zhu, K. W. Borelli, J. R. Greenwood, et al. Docking covalent inhibitors: a parameter
free approach to pose prediction and scoring. J Chem Inf Model. 2014; 54 : 1932-1940.
24. Ehmke V, Winkler E, Banner DW, Haap W, et al. Potent and Selective Inhibition of
Cysteine Proteases from Plasmodium falciparum and Trypanosoma brucei.
ChemMedChem. 2013; 8: 967–975.
25. Giroud M, Ivkovic V, Martignoni M, Fleuti M, et al. Inhibition of the Cysteine Protease
Human Cathepsin L by Triazine Nitriles: Amide-Heteroarene π-Stacking Interactions
and Chalcogen Bonding in the S3 Pocket. ChemMedChem. 2017;12:257-270.
26. Lalmanach G, Diot E, Godat E, Lecaille F, Hervé-Grépinet V. Cysteine cathepsins and
caspases in silicosis. Biol. Chem. 2006; 387: 863-870.
27. Taggart C, Mall MA, Lalmanach G, Cataldo D, et al. Taggart C, Mall MA, Lalmanach G,
Cataldo D, et al. Protean proteases: at the cutting edge of lung diseases. Eur Resp J.
2017 ; 49: 1501200.
28. Serveau-Avesque C, Ferrer-Di Martino M, Hervé-Grépinet V, Hazouard E, et al. Active
cathepsins B, H, K, L and S in human inflammatory bronchoalveolar lavage fluids. Biol
Cell. Biol. Cell. 2006; 98: 15-22.
29. Quraishi O, Storer AC. Identification of Internal Autoproteolytic Cleavage Sites within
the Prosegments of Recombinant Procathepsin B and Procathepsin S contribution of a
plausible unimolecular autoproteolytic event for the processing of zymogens belonging
to the papain family. J Biol Chem. 2001;276:8118-8124.
30. Brömme D, Panwar P, Turan S. Cathepsin K osteoporosis trials, pycnodysostosis and
mouse deficiency models: commonalities and differencesExpert Opin Drug Discov.
2016; 11:457-472.
31. Lecaille F, Weidauer E, Juliano MA, Brömme D. Lalmanach G. Probing cathepsin K
activity with a selective substrate spanning its active site.Biochem J. 2003; 375:307-
312.
32. Sage J, Mallèvre F, Barbarin-Costes F, Samsonov SA, Gehrckeet J-P al. Binding of
chondroitin 4-Sulfate to cathepsin S regulates Its enzymatic activity. Biochemistry.
2013; 52:6487-6498.

Graphical Abstract

Highlights
-
-
17 novel 1,3,5-triazines analogs were synthesized and evaluated
Human Cat B, K, L and S with endopeptidase activity are targeted by those
compounds
-
-
7c was the most active and selective Cat S inhibitor with an IC50 value of 4 nM
Molecular docking studies of 7c was performed