Zur Totalsynthese von Human-Sekretin

Article abstract of DOI:10.1007/BF00819525

The synthesis of the heptacosapeptide amide with the primary structure of Human-secretin is described.For this purpose 7 fragments were designed, i.e.H-Gly-Leu-Val-NH2 <25-27b>, Z-Arg(Z2)-Leu-Leu-Gln-OH <21-24>, Z-Arg(Z2)-Leu-Gln-OH <18-20>, Z-Arg(Z2)-Glu(OtBu)-Gly-Ala-OH <14-17>, Z-Arg(Z2)-Leu-OH <12-13>, Z-Thr(tBu)-Ser(tBu)-Glu(OtBu)-Leu-Ser(tBu)-OH <7-11>, Adoc-His(Adoc)-Ser(tBu)-Asp(OtBu)-Gly-Thr(tBu)-Phe-OH <1-6>; these fragments were consequently assembled to the overall protected total sequence using the Wuensch/Weygand-method with dicyclohexylcarbodiimide.After deprotection by exposure to trifluoroacetic acid in presence of 1,2-ethanediol and water as scavenger, the islated crude product was purified by column chromatography on CM-Sepharose, fast flow.This synthesized Human-secretin showed the full biological activity in comparison to Porcine-secretin. Key words: Gastrointestinal hormone; Human-secretin: synthetic peptide factors.