REGIOSELECTIVE SYNTHESIS OF TRISACCHARIDES BY USE OF A REVERSED HYDROLYSIS ACTIVITY OF α- AND β-D-GALACTOSIDASE

Article abstract of DOI:10.1016/0008-6215(89)84029-7

Incubation of a solution containing D-galactose and a high concentration of sucrose in the presence of α-D-galactosidase from M. vinacea afforded raffinose and planteose in a ratio of ca. 3:2.On the other hand, circulation of a solution of D-galactose and sucrose through a reaction system consisting of columns, in series, of immobilized α-D-galactosidase and activated carbon gave only raffinose, eluted from the activated carbon column in a 17.6percent yield.Similarly, only isoraffinose was obtained in 10.6percent yield by the continuous method using an immobilized column of β-D-galactosidase from E. coli.