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Fig. 6. Hydrolysis products of 10 mg/mL oat spelt xylan by XylX at the 72nd hour.
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product of oat spelt xylan, with 1.224 mg/mL produced after 72 h of
hydrolysis. Of the total hydrolysis products, 25.42% were xylotriose.
The late appearance of xylose after the 12th hour correlated with
the observation of trace relative activities for p-nitrophenyl--d-
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by XylX hydrolysis from birchwood xylan than from oat spelt xylan,
as shown in Table 3. Xylotriose was also the major hydrolytic
product of oat spelt xylan, with 2.337 mg/mL produced after 72 h
of hydrolysis. A total of 28.72% of the hydrolysis products were
xylotriose. The above result demonstrates that XylX is an endo-type
xylanase. It also validates that XylX can be employed to produce
xylo-oligomers, which are increasingly important prebiotic food
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A high temperature, alkaline environment is best for isolat-
ing microorganisms and associated enzymes for specific industrial
applications. This concept is also proposed in a recent study [37].
This is the first report of a 41-kDa xylanase identified from Paeni-
bacillus sp. with only 73% homology to the next closest sequence in
NCBI database. Wide pH adaptability, high thermostability, specific
xylanase activity and a complete lack of cellulase activity render
XylX an excellent candidate for many potential applications, espe-
cially kraft pulp bleaching pretreatment. The crude xylanase from
Paenibacillus sp. BL-11 was already proved successful for applica-
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Acknowledgements
We thank Ms. Mei-Jane Fang for her technical assistance in DNA
sequencing. We thank the support of the grants from NSC, Taiwan,
ROC here. We also thank the funding support by National Science
Council, Taiwan, ROC for two grants: 92-2313-B-002-125 and 94-
2313-B-002-083.
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