Preparation of the radioiodinated histamine amide of 4-O-(carboxypropyl)-diethylstilbestrol

Article abstract of DOI:10.1002/jlcr.2580160317

The mixed anhydride synthesis provides a route to an iodinated form of the synthetic estrogen diethylstilbestrol (DES). Labelling of histamine by the oxidative Chloramine-T method prior to coupling to 4-O-(carboxypropyl)-diethylstilbestrol minimizes radiation damage to the sensitive DES derivative. The radioiodinated derivative has demonstrated binding to an anti-DES serum, thus making possible a potentially useful iodine-based radioimmunoassay for DES.