An assay for urinary estriol-16α-glucuronide based on antibody-enhanced chemiluminescence

Article abstract of DOI:10.1016/0039-128X(80)90029-X

An immunoassay for estriol-16α-glucuronide in pregnancy urine is described that utilizes antibody-enhanced chemiluminescence. The steroid glucuronide was covalently conjugated with the chemiluminescent marker aminobutyl-ethyl-isoluminol. The light yield of this conjugate upon oxidation was augmented by specific antibody, and this effect was inhibited by addition of the homologous steroid glucuronide (10-100 pg) in a dose-dependent manner. The assay does not require separation of bound and free ligand and proved satisfactory with respect to sensitivity, precision and accuracy. Assay results obtained by radioimmunoassay and chemiluminescence immunoassay were in good agreement (r=0.98; n=25). An immunoassay for estriol-16 alpha-glucuronide in urine, for pregnancy determinations, that uses antibody-enhanced chemiluminescence is described. Glucuronide was convalently conjugated with amino butyl-ethyl-isoluminol, a chemiluminescent marker. A specific antibody was used to augment the light yield of this conjugate upon oxidation, and this effect was inhibited by addition of the monologous steroid glucuronide (10-100 pgm) dose-dependently. The assay does not require separation of bound and free ligand, and its sensitivity, precision, and accuracy were satisfactory. Good agreement was found between assay results obtained by radioimmunoassay and the chemiliminescence immunoassay (r=.98; n=25).