Glycosidase-catalysed Oligosaccharide Synthesis: Preparation of the N-Acetylchitooligosaccharides Penta-N-acetylchitopentaose and Hexa-N-acetylchitohexaose using the β-N-Acetylhexosaminidase of Aspergillus oryzae.

Article abstract of DOI:10.1016/0957-4166(95)00370-5

Using a crude β-N-acetylhexosaminidase from Aspergillus oryzae both tri-N-acetylchitotriose (GlcNAc)3 (1,n=1) and tetra-N-acetylchitotetraose (GlcNAc4) (1,n=2) act respectively as both glycosyl donor and glycosyl acceptor to give product mixtures containing significant quantities of the corresponding penta- and hexasaccharides <1 (n=3) and 1 (n=4), respectively> which are readily isolated and purified by charcoal-Celite chromatography.