H
M. Liu et al.
1H NMR and 2D NMR
4 mg mLꢁ1 in PBS was added to 150 mL of the cell cultures in the
96-microwell flat-bottom plate for 24 h incubation at 378C.
Then, the solutions in the plates were centrifuged (1000 g for
5 min.), and the MTT-containing culture medium was removed.
The precipitated formazan was dissolved in 120 mL DMSO.
Results were read within 15 min using a spectrometer at 490 nm.
Finally, the means of the triplicates were calculated. Cell inhi-
bition rate was expressed as a percentage of the control samples.
1H NMR spectra of the LU/CD inclusion complexes and LU
monomer were obtained using a Bruker Avance DRX spec-
trometer at 500 MHz and 298 K using D2O and [D6]DMSO,
respectively. Data were collected without an external reference
in order to avoid potential interactions with the CDs. ROESY
data were obtained on a Bruker Avance DRX500 instrument and
were implemented in D2O.
XRD
Supplementary Material
Stoichiometry and speciation plots; absorption, 1H NMR
and ROESY spectra, and SEM images for various inclusion
complexes are available on the Journal’s website.
The XRD patterns were recorded on a PHI 5000 VersaProbe II
˚
temperature, and a scanning speed was 48 minꢁ1. Powder
samples were mounted on a vitreous sample holder and scanned
with a step size of 2y 0.028 in the 2y range of 5–658.
using Cu-Ka radiation (l 1.5460 A, 40 kV, 100 mA) at room
Acknowledgements
We gratefully acknowledge support from the National Natural Science
Foundation of China (NNSFC) (Nos 21362016, 21361014, and 21302074).
DSC
DSC measurements were performed on
a NETZSCH
STA449F3, using a heating rate of 108C minꢁ1 in the temper-
ature range of 30–4608C in a dynamic nitrogen atmosphere
(flow rate ¼ 100 mL minꢁ1).
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Human hepatoma cell line (Hep G2) is one of the most common
experimental models used in in vitro studies.[40] Thus, we cul-
tured Hep G2 cells at 4 ꢀ 104 mLꢁ1 in RPMI-1640 medium
containing 10 % heat-inactivated fetal bovine serum supplement
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Next, 50 mL of MTT stock solution, at a concentration of