A CHEMICAL 5'-PHOSPHORYLATION OF OLIGODEOXYRIBONUCLEOTIDES THAT CAN BE MONITORED BY TRITYL CATION RELEASE

DOI: 10.1016/S0040-4039(00)85043-1

Source and publish data:

Tetrahedron Letters p. 4705 - 4708 (1986)

Update date:2022-08-04

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Authors:

Horn, Thomas

Urdea, Mickey S.

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Article abstract of DOI:10.1016/S0040-4039(00)85043-1

A new phosphoramidite-derived reagent, (2-cyanoethoxy)-2-(2'-O-4,4'-dimethoxytrityloxyethylsulfonyl)ethoxy-N,N-diisopropylaminophosphine, for the 5'-phosphorylation of oligodeoxyribonucleotides has been developed.Phosphorylation efficiency can be determined by the release of 4,4'-dimethoxytrityl cation in acid.

Full text of DOI:10.1016/S0040-4039(00)85043-1

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