Tetrahedron Letters p. 4705 - 4708 (1986)
Update date:2022-08-04
Topics:
Horn, Thomas
Urdea, Mickey S.
A new phosphoramidite-derived reagent, (2-cyanoethoxy)-2-(2'-O-4,4'-dimethoxytrityloxyethylsulfonyl)ethoxy-N,N-diisopropylaminophosphine, for the 5'-phosphorylation of oligodeoxyribonucleotides has been developed.Phosphorylation efficiency can be determined by the release of 4,4'-dimethoxytrityl cation in acid.
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