Steric Course of N5,N10-Methylenetetrahydrofolate Formation from Glycine by the Glycine Cleavage System in E. coli

Article abstract of DOI:10.1021/ja00329a046

The steric course of the conversion of glycine to 5,10-methylenetetrahydrofolate (5,10-CH2-H4folate) by the glycine cleavage system in E. coli has been elucidated. 2-H_re of glycine is the precursor of 11-H_re of 5,10-CH2-H4folate, and 2-H_si of glycine is the precursor of 11-H_si of 5,10-CH2-H4folate.The overall transformation is at least 78percent stereospecific (possibly higher).